AB192607

Anti-DAP13/NDUFA12 antibody [EPR15867]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal DAP13/NDUFA12 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

DAP13, NDUFA12, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12, 13 kDa differentiation-associated protein, Complex I-B17.2, NADH-ubiquinone oxidoreductase subunit B17.2, CI-B17.2, CIB17.2

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

Immunofluorescent analysis of Hela cells permeabilised in 0.1% Triton X-100 and fixed in 4% paraformaldehyde labeling DAP13/NDUFA12 with ab192607 at 1/50 (5.9 μg/ml) and Goat anti rabbit IgG(Alexa Fluor® 488) ab150077. Counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

Immunohistochemical analysis of formalin fixed paraffin embedded Human pancreas labeling DAP13/NDUFA12 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling DAP13/NDUFA12 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

All lanes:

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/1000 dilution

Lane 1:

Human fetal heart at 20 µg

Lane 2:

Human skeletal muscle at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 17 kDa

false

Supplier Data

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

All lanes:

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/5000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 7 kDa

false

Supplier Data

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

All lanes:

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

false

Supplier Data

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

All lanes:

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/5000 dilution

All lanes:

Rat muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

false

Carrier free

Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15867

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/300 - 1/600", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/300 - 1/600", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

See full target information NDUFA12

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neoplasma 68:262-272 PubMed33147052

2020

Upregulation of microRNA-650 by PBX1 is correlated with the development of Helicobacter pylori-associated gastric carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Junzheng Liu,Lei Wang,Jie Li,Yu Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DAP13/NDUFA12 antibody [EPR15867]

Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (AB192607)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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