Rabbit Recombinant Monoclonal DAP13/NDUFA12 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (AB251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--western-blot-img57351.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (AB251142)")
![Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (AB251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--immunocytochemistry-immunofluorescence-img108327.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (AB251142)")
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (AB251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--western-blot-img125142.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (AB251142)")
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (AB251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--western-blot-img12539.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (AB251142)")
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (AB251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--western-blot-img150797.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (AB251142)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Alternative names
DAP13, NDUFA12, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12, 13 kDa differentiation-associated protein, Complex I-B17.2, NADH-ubiquinone oxidoreductase subunit B17.2, CI-B17.2, CIB17.2
Recommended products
Rabbit Recombinant Monoclonal DAP13/NDUFA12 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR15867
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251142 is the carrier-free version of Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--western-blot-img57351.jpg "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)")
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
This data was developed using Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607) at 1/1000 dilution
Lane 1: Human fetal heart at 20 µg
Lane 2: Human skeletal muscle at 20 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 17 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--immunocytochemistry-immunofluorescence-img108327.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)")
Immunocytochemistry/ Immunofluorescence - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
This data was developed using Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Hela cells permeabilised in 0.1% Triton X-100 and fixed in 4% paraformaldehyde labeling DAP13/NDUFA12 with Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607 at 1/50 (5.9 μg/ml) and Goat anti rabbit IgG(Alexa Fluor® 488) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077. Counterstained with DAPI.
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--western-blot-img125142.jpg "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)")
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
This data was developed using Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607) at 1/5000 dilution
Lane 1: Human fetal kidney lysate at 10 µg
Lane 2: Human fetal liver lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Observed band size: 7 kDa
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--western-blot-img12539.jpg "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)")
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
This data was developed using Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607) at 1/5000 dilution
All lanes: Rat muscle tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
![Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--western-blot-img150797.jpg "Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)")
Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
This data was developed using Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] (Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 10 µg
Lane 2: Rat brain tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119944.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
This data was developed using Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin fixed paraffin embedded Human pancreas labeling DAP13/NDUFA12 with Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114068.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
This data was developed using Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling DAP13/NDUFA12 with Anti-DAP13/NDUFA12 antibody [EPR15867] ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119944.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142), expandable thumbnail](https://content.abcam.com/products/images/dap13-ndufa12-antibody-epr15867-bsa-and-azide-free-ab251142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114068.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)")
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