Mouse Recombinant Monoclonal DAP3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (AB302890), expandable thumbnail](https://content.abcam.com/products/images/dap3-antibody-10-dap3-bsa-and-azide-free-ab302890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401989.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (AB302890)")
![Western blot - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (AB302890), expandable thumbnail](https://content.abcam.com/products/images/dap3-antibody-10-dap3-bsa-and-azide-free-ab302890--western-blot-img402119.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (AB302890)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (AB302890), expandable thumbnail](https://content.abcam.com/products/images/dap3-antibody-10-dap3-bsa-and-azide-free-ab302890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401991.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (AB302890)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (AB302890), expandable thumbnail](https://content.abcam.com/products/images/dap3-antibody-10-dap3-bsa-and-azide-free-ab302890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401990.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (AB302890)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Mouse | Tested | Tested | Not recommended |
| Rat | Tested | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Involved in mediating interferon-gamma-induced cell death.
Alternative names
MRPS29, DAP3, Small ribosomal subunit protein mS29, Death-associated protein 3, Ionizing radiation resistance conferring protein, MRP-S29, S29mt, DAP-3
Recommended products
Mouse Recombinant Monoclonal DAP3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 10-DAP3
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890), expandable thumbnail](https://content.abcam.com/products/images/dap3-antibody-10-dap3-bsa-and-azide-free-ab302890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401989.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890)
This data was developed using Anti-DAP3 antibody [10/DAP3] ab302889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling DAP3 with Anti-DAP3 antibody [10/DAP3] ab302889 at 1/100 (10.44 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on the mitochondrion in human colon. The section was incubated with Anti-DAP3 antibody [10/DAP3] ab302889 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Western blot - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890), expandable thumbnail](https://content.abcam.com/products/images/dap3-antibody-10-dap3-bsa-and-azide-free-ab302890--western-blot-img402119.jpg "Western blot - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890)")
Western blot - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890)
This data was developed using Anti-DAP3 antibody [10/DAP3] ab302889, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: 48 seconds
All lanes: Western blot - Anti-DAP3 antibody [10/DAP3] (Anti-DAP3 antibody [10/DAP3] ab302889) at 1/1000 dilution
Lane 1: Human kidney tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
Lane 3: U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg
Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 20 µg
Lane 5: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Lane 6: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 7: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution
Observed band size: 40 kDa
Exposure time: 48s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890), expandable thumbnail](https://content.abcam.com/products/images/dap3-antibody-10-dap3-bsa-and-azide-free-ab302890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401991.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890)
This data was developed using Anti-DAP3 antibody [10/DAP3] ab302889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling DAP3 with Anti-DAP3 antibody [10/DAP3] ab302889 at 1/100 (10.44 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on the mitochondrion in rat colon. The section was incubated with Anti-DAP3 antibody [10/DAP3] ab302889 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890), expandable thumbnail](https://content.abcam.com/products/images/dap3-antibody-10-dap3-bsa-and-azide-free-ab302890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401990.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP3 antibody [10/DAP3] (BSA and Azide free) (ab302890)
This data was developed using Anti-DAP3 antibody [10/DAP3] ab302889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling DAP3 with Anti-DAP3 antibody [10/DAP3] ab302889 at 1/100 (10.44 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on the mitochondrion in mouse colon. The section was incubated with Anti-DAP3 antibody [10/DAP3] ab302889 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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