Anti-DAP3 antibody [42C617]

• WB

Unknown

Western blot - Anti-DAP3 antibody [42C617] (AB11928)

All lanes:

Western blot - Anti-DAP3 antibody [42C617] (ab11928) at 2 µg/mL

All lanes:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 46 kDa

Observed band size: 43 kDa

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• WB

CiteAb

Western blot - Anti-DAP3 antibody [42C617] (AB11928)

DAP3 western blot using anti-DAP3 antibody [42C617] ab11928. Publication image and figure legend from Hornig-Do, H. T., Montanari, A., et al., 2014, EMBO Mol Med, PubMed 24413189.

ab11928 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab11928 please see the product overview.

In each case aaRS overexpression was induced by 3 days tetracycline (Tet) treatment, indicated by + or − symbols. All data and images represent a minimum of 3 independent experiments.Cell growth under glycolytic or respiratory conditions with or without overexpression of aaRS. Equal numbers of 143B.206 Rho+, T1, T1V1 and T1L1 cells were seeded in medium containing either glucose or galactose as the sole sugar source. The extent of growth and viability of each cell line with or without aaRS overexpression was determined at 72 h using the neutral red assay. Statistically significant comparisons for uninduced and induced cells are indicated with * ( P = 0.016), ** ( P = 0.0086) n = 3.Overexpression of aaRS. Mitochondrial proteins were isolated from uninduced and aaRS overexpressors. Mitochondrial lysates from T1V1 (100 μg) and T1L1 (50 μg) were subjected to western blot analysis using antibodies against VARS2L or FLAG to confirm overexpression. Mitochondrial ribosomal protein DAP3 was used to confirm equal loading.Analysis of steady state levels of OXPHOS proteins following aaRS induction. Cell lysates (15 μg) from 143B.206 Rho+ and T1V1 and T1L1 cells with or without induction were subjected to western blot analysis. Membranes were probed with antibodies directed against OXPHOS proteins and porin as a loading control.Blue Native-PAGE analysis of respiratory chain complex levels. Mitochondria (25 μg) from uninduced and induced T1V1 and T1L1 cells were solubilised for BN-PAGE. Subsequent western blot analysis used antibodies against complex I (NDUFA9), complex IV (COX4), complex III (CORE2) and as a non-mitochondrially encoded control, complex II (SDHA).In gel enzyme activity assay of respiratory chain complexes. BN-PAGE was performed on solubilised mitochondria (50 μg) from uninduced and induced T1V1 and T1L1 cells. Enzyme activities for complexes I, IV and II were examined.

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