Anti-DAP3 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DAP3 antibody (AB2637)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling DAP3 with ab2637 at 10 μg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor^® 488-conjugated secondary antibody at 2 μg/ml. DAPI nuclear counterstain was used.

Negative control : Unimmunized goat IgG (10 μg/ml) followed by an Alexa Fluor^® 488-conjugated secondary antibody (2 μg/ml).

• WB

Supplier Data

Western blot - Anti-DAP3 antibody (AB2637)

Detected using chemiluminescence.

All lanes:

Western blot - Anti-DAP3 antibody (ab2637) at 0.3 µg/mL

All lanes:

Human kidney tissue lysate in RIPA buffer at 30 µg

Predicted band size: 46 kDa

false

• WB

Supplier Data

Western blot - Anti-DAP3 antibody (AB2637)

Level of knock-down relative to actin expression level determined by RT-PCR.

All lanes:

Western blot - Anti-DAP3 antibody (ab2637) at 1 µg/mL

Lane 1:

HeLa cell lysate in RIPA buffer - control at 35 µg

Lane 2:

HeLa cell lysate in RIPA buffer - after siRNA mediated DAP3 knock-down expression at 35 µg

Predicted band size: 46 kDa

false

• WB

Supplier Data

Western blot - Anti-DAP3 antibody (AB2637)

Detected using chemiluminescence.

All lanes:

Western blot - Anti-DAP3 antibody (ab2637) at 0.3 µg/mL

Lane 1:

HeLa cell lysate in RIPA buffer at 30 µg

Lane 2:

HepG2 cell lysate in RIPA buffer at 30 µg

Predicted band size: 46 kDa

false