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AB137044

Anti-DARC antibody [EPR5205]

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(8 Publications)

Rabbit Recombinant Monoclonal DARC antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

CD234, DARC, FY, GPD, ACKR1, Atypical chemokine receptor 1, Duffy antigen receptor for chemokines, Duffy antigen/chemokine receptor, Duffy blood group antigen, Fy glycoprotein, Glycoprotein D, Plasmodium vivax receptor, GpFy

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (AB137044)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (AB137044)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling DARC with Purified ab137044 at 1 : 500 dilution (1.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (AB137044)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (AB137044)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling DARC with ab137044 at 1/100 dilution.

This image was generated using the unpurified version of the product.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-DARC antibody [EPR5205] (AB137044)
  • IP

Unknown

Immunoprecipitation - Anti-DARC antibody [EPR5205] (AB137044)

ab137044 (purified) at 1 : 30 dilution (2μg) immunoprecipitating DARC in TF-1 whole cell lysate.
Lane 1 (input) : TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10μg
Lane 2 (+) : ab137044 & TF-1 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab137044 in TF-1 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-DARC antibody [EPR5205] (ab137044)

Predicted band size: 35 kDa

false

Western blot - Anti-DARC antibody [EPR5205] (AB137044)
  • WB

Unknown

Western blot - Anti-DARC antibody [EPR5205] (AB137044)

This image was generated using the unpurified version of the product.

All lanes:

Western blot - Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Human tonsil lysate at 10 µg

Lane 4:

TF 1 lysate at 10 µg

Lane 5:

K562 lysate at 10 µg

Secondary

All lanes:

HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 35 kDa

false

Western blot - Anti-DARC antibody [EPR5205] (AB137044)
  • WB

Lab

Western blot - Anti-DARC antibody [EPR5205] (AB137044)

All lanes:

Western blot - Anti-DARC antibody [EPR5205] (ab137044) at 1/2000 dilution

All lanes:

Human kidney lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 35 kDa

Observed band size: 40 kDa

false

Western blot - Anti-DARC antibody [EPR5205] (AB137044)
  • WB

Lab

Western blot - Anti-DARC antibody [EPR5205] (AB137044)

All lanes:

Western blot - Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution

All lanes:

Human stomach lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 35 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5205

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250.</strong></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1 and TARC. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms : when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes; when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels.. (Microbial infection) Acts as a receptor for the malaria parasite Plasmodium vivax.. (Microbial infection) Acts as a receptor for the malaria parasite Plasmodium knowlesi.
See full target information ACKR1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3348 PubMed40199901

2025

Spatially resolved atlas of breast cancer uncovers intercellular machinery of venular niche governing lymphocyte extravasation.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wang,Zhanyu Wang,Qijun Liao,Pei Yuan,Junpu Mei,Yin Zhang,Chao Wu,Xiyu Kang,Sufei Zheng,Chenxuan Yang,Jiaxiang Liu,Qingyao Shang,Jiangtao Li,Bingning Wang,Liangyu Li,Hui Liu,Weining Hu,Zhensheng Dong,Jie Zhao,Linying Wang,Tao Liu,Yusheng Den,Chengrui Wang,Lijuan Han,Qianjun Chen,Huanming Yang,Xun Xu,Jie He,Zhen Yue,Nan Sun,Xiaodong Fang,Jianming Ying

Cell reports. Medicine 6:102024 PubMed40107247

2025

Immune-featured stromal niches associate with response to neoadjuvant immunotherapy in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Tong Liu,Hai-Ming Liu,Jian-Gang Ren,Wei Zhang,Xin-Xin Wang,Zi-Li Yu,Qiu-Yun Fu,Xue-Peng Xiong,Jun Jia,Bing Liu,Gang Chen

iScience 27:111052 PubMed39635131

2024

Red blood cell signaling is functionally conserved in invasion.

Applications

Unspecified application

Species

Unspecified reactive species

James Jia Ming Yong,Xiaohong Gao,Prem Prakash,Jing Wen Ang,Soak Kuan Lai,Ming Wei Chen,Jason Jun Long Neo,Julien Lescar,Hoi Yeung Li,Peter R Preiser

Arteriosclerosis, thrombosis, and vascular biology 43:2143-2164 PubMed37706320

2023

Single-Cell RNA Sequencing of Coronary Perivascular Adipose Tissue From End-Stage Heart Failure Patients Identifies Macrophage Subpopulation as a Target for Alleviating Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengxia Fu,Songren Shu,Zhiming Peng,Xiaorui Liu,Xiao Chen,Zhiwei Zeng,Yicheng Yang,Hao Cui,Ruojin Zhao,Xiaohu Wang,Leilei Du,Min Wu,Wei Feng,Jiangping Song

Transfusion 63:586-600 PubMed36752125

2023

Different-sized extracellular vesicles derived from stored red blood cells package diverse cargoes and cause distinct cellular effects.

Applications

Unspecified application

Species

Unspecified reactive species

Joo-Yeun Oh,Marisa B Marques,Xin Xu,Jindong Li,Kristopher R Genschmer,Edward Phillips,Melissa F Chimento,James Mobley,Amit Gaggar,Rakesh P Patel

Arteriosclerosis, thrombosis, and vascular biology 41:1408-1427 PubMed33626908

2021

Single-Cell Transcriptomic Atlas of Different Human Cardiac Arteries Identifies Cell Types Associated With Vascular Physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Zhan Hu,Wendao Liu,Xiumeng Hua,Xiao Chen,Yuan Chang,Yiqing Hu,Zhenyu Xu,Jiangping Song

Cardiovascular research 106:478-87 PubMed25858253

2015

Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice.

Applications

IHC-Fr

Species

Mouse

Wuzhou Wan,Qian Liu,Michail S Lionakis,Ana Paula M P Marino,Stasia A Anderson,Muthulekha Swamydas,Philip M Murphy

Journal of immunology (Baltimore, Md. : 1950) 193:1416-26 PubMed24981451

2014

CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines.

Applications

Unspecified application

Species

Unspecified reactive species

Laila A Al-Alwan,Ying Chang,Simon Rousseau,James G Martin,David H Eidelman,Qutayba Hamid
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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