AB40802

Anti-DARPP32 antibody [EP721Y]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal DARPP32 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 4 publications.

View Alternative Names

DARPP32, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP721Y] (AB40802)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP721Y] (AB40802)

ab40802 staining DARPP32 in SH-SY5Y (human neuroblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab40802 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1 : Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2 : Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (AB40802)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (AB40802)

Immunohistochemical analysis human gastric adenocarcinoma using ab40802. (dilution 1 : 250)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (AB40802)

ab40802 staining DARPP32 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

Negative control 1 : PBS in place of primary antibody.

Unknown

Immunoprecipitation - Anti-DARPP32 antibody [EP721Y] (AB40802)

ab40802 immunoprecipitating DARPP32. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/50 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

Lane 1 : Human fetal forebrain whole cell lysate (10ug)
Lane 2 : Human fetal forebrain whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab40802 in Human fetal forebrain whole cell lysate

All lanes:

Immunoprecipitation - Anti-DARPP32 antibody [EP721Y] (ab40802)

Predicted band size: 23 kDa

Observed band size: 32 kDa

false

Lab

Western blot - Anti-DARPP32 antibody [EP721Y] (AB40802)

All lanes:

Western blot - Anti-DARPP32 antibody [EP721Y] (ab40802) at 1/50000 dilution

All lanes:

Human brain tissue lysates. at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 23 kDa

Observed band size: 32 kDa

false

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-DARPP32 antibody [EP721Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-DARPP32 antibody [EP721Y]
Carrier free

Anti-DARPP32 antibody [EP721Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP721Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/50000", "WB-species-notes": "Unpurified format: 1/5,000 - 1/20,000", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.11% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitor of protein-phosphatase 1.

See full target information PPP1R1B

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 12:1552345 PubMed40969344

2025

Immunohistochemical characterization and potential prognostic relevance of dopamine signaling in canine pulmonary adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Alana R Kuzmik,Davis M Seelig,Luke H Hoeppner,Aaron Rendahl,Hannah Able,Amber Wolf-Ringwall,Jessica Lawrence

Nature communications 13:27 PubMed35031607

2022

Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants.

Applications

Unspecified application

Species

Unspecified reactive species

Bret Sanders,Daniel D'Andrea,Mark O Collins,Elliott Rees,Tom G J Steward,Ying Zhu,Gareth Chapman,Sophie E Legge,Antonio F Pardiñas,Adrian J Harwood,William P Gray,Michael C O'Donovan,Michael J Owen,Adam C Errington,Derek J Blake,Daniel J Whitcomb,Andrew J Pocklington,Eunju Shin

Frontiers in cellular neuroscience 15:742763 PubMed34658796

2021

Mislocalization of Nucleocytoplasmic Transport Proteins in Human Huntington's Disease PSC-Derived Striatal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Jenny Lange,Alison Wood-Kaczmar,Aneesa Ali,Sahar Farag,Rhia Ghosh,Jennifer Parker,Caroline Casey,Yumiko Uno,Akiyoshi Kunugi,Patrizia Ferretti,Ralph Andre,Sarah J Tabrizi

Pharmaceutics 11: PubMed31614758

2019

A Combinatorial Cell and Drug Delivery Strategy for Huntington's Disease Using Pharmacologically Active Microcarriers and RNAi Neuronally-Committed Mesenchymal Stromal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie M André,Gaëtan J Delcroix,Saikrishna Kandalam,Laurence Sindji,Claudia N Montero-Menei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DARPP32 antibody [EP721Y]

Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP721Y] (AB40802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (AB40802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (AB40802)

Immunoprecipitation - Anti-DARPP32 antibody [EP721Y] (AB40802)

Western blot - Anti-DARPP32 antibody [EP721Y] (AB40802)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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