Rabbit Polyclonal DARPP32 phospho S137 antibody. Suitable for WB and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Rat Ppp1r1b phospho S137.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 10% BSA, 0.87% Sodium chloride, 0.24% HEPES- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Rat Ppp1r1b phospho S137. The exact immunogen used to generate this antibody is proprietary information. Database link Q6J4I0
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Predicted |
| Rat | Tested |
| Chicken | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Primates | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Chicken, Cow, Dog, Primates | Dilution info - | Notes - |
Target data
Function
Inhibitor of protein-phosphatase 1.
Alternative names
Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, Ppp1r1b
Recommended products
Rabbit Polyclonal DARPP32 phospho S137 antibody. Suitable for WB and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Rat Ppp1r1b phospho S137.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 10% BSA, 0.87% Sodium chloride, 0.24% HEPES- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Rat Ppp1r1b phospho S137. The exact immunogen used to generate this antibody is proprietary information. Database link Q6J4I0
- Purification technique
- Affinity purification Immunogen
- Specificity
Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Ser137. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
- Concentration
- Purification notes
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
Western blot - Anti-DARPP32 (phospho S137) antibody (ab254065)
All lanes: Western blot - Anti-DARPP32 (phospho S137) antibody (ab254065) at 1/1000 dilution
Lane 1: Rat striatal tissue lysate
Lane 2: Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Predicted band size: 23 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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