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AB254063

Anti-DARPP32 (phospho T34) antibody

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(2 Publications)

Rabbit Polyclonal DARPP32 phospho T34 antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Rat Ppp1r1b pT34.

View Alternative Names

Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, Ppp1r1b

1 Images
Western blot - Anti-DARPP32 (phospho T34) antibody (AB254063)
  • WB

Supplier Data

Western blot - Anti-DARPP32 (phospho T34) antibody (AB254063)

All lanes:

Western blot - Anti-DARPP32 (phospho T34) antibody (ab254063) at 1/1000 dilution

Lane 1:

Rat striatal lysate

Lane 2:

Rat striatal lysate with treatment with lambda-phosphatase (1200 units for 30 minutes)

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Rat Ppp1r1b pT34. The exact immunogen used to generate this antibody is proprietary information.

Q6J4I0

Specificity

Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr34. Immunolabeling is completely eliminated by treatment with λ-Ptase.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Storage buffer
pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 10% BSA, 0.87% Sodium chloride, 0.24% HEPES
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitor of protein-phosphatase 1.
See full target information Ppp1r1b pT34
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:21163 PubMed38036593

2023

Unravelling transcriptomic complexity in breast cancer through modulation of DARPP-32 expression and signalling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Behnaz Saidy,Richa Vasan,Rosie Durant,Megan-Rose Greener,Adelynn Immanuel,Andrew R Green,Emad Rakha,Ian Ellis,Graham Ball,Stewart G Martin,Sarah J Storr

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 20:1081-1108 PubMed37079191

2023

Ferulic Acid Improves Synaptic Plasticity and Cognitive Impairments by Alleviating the PP2B/DARPP-32/PP1 Axis-Mediated STEP Increase and Aβ Burden in Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yacoubou Abdoul Razak Mahaman,Fang Huang,Maibouge Tanko Mahamane Salissou,Mohamed Bassirou Moukeila Yacouba,Jian-Zhi Wang,Rong Liu,Bin Zhang,Hong-Lian Li,Feiqi Zhu,Xiaochuan Wang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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