AB51114

Anti-DARPP32 (phospho T75) antibody

Name: Anti-DARPP32 (phospho T75) antibody (ab51114) | Abcam
Brand: Abcam Limited
SKU: AB51114
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal DARPP32 phospho T75 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R1B phospho T75 aa 1-100.

View Alternative Names

DARPP32, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 (phospho T75) antibody (AB51114)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 (phospho T75) antibody (AB51114)

ab51114 staining human DARPP32 in human brain tissue by immunohistochemistry using paraffin embedded tissue.

Unknown

Western blot - Anti-DARPP32 (phospho T75) antibody (AB51114)

All lanes:

Western blot - Anti-DARPP32 (phospho T75) antibody (ab51114) at 1 µg/mL

Lane 1:

MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 23 kDa

Observed band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PPP1R1B phospho T75 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UD71

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitor of protein-phosphatase 1.

See full target information PPP1R1B phospho T75

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed37893051

2023

Extrapyramidal Side Effects with Chronic Atypical Antipsychotic Can Be Predicted by Labeling Pattern of FosB and phosphoThr-DARPP-32 in Nucleus Accumbens.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia G Prieto,Maria Camila Almeida,João C S Silva,Elaine Del-Bel,Marcela B Echeverry

Communications biology 5:451 PubMed35551287

2022

Microfluidics delivery of DARPP-32 into HeLa cells maintains viability for in-cell NMR spectroscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Sciolino,Anna Liu,Leonard Breindel,David S Burz,Todd Sulchek,Alexander Shekhtman

Molecular metabolism 51:101241 PubMed33933677

2021

Dopamine D2 receptor agonist, bromocriptine, remodels adipose tissue dopaminergic signalling and upregulates catabolic pathways, improving metabolic profile in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

G Tavares,D Marques,C Barra,D Rosendo-Silva,A Costa,T Rodrigues,P Gasparini,B F Melo,J F Sacramento,R Seiça,S V Conde,P Matafome

Experimental neurobiology 27:103-111 PubMed29731676

2018

Genetic Ablation of EWS RNA Binding Protein 1 (EWSR1) Leads to Neuroanatomical Changes and Motor Dysfunction in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yeojun Yoon,Hasang Park,Sangyeon Kim,Phuong T Nguyen,Seung Jae Hyeon,Sooyoung Chung,Hyeonjoo Im,Junghee Lee,Sean Bong Lee,Hoon Ryu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DARPP32 (phospho T75) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 (phospho T75) antibody (AB51114)

Western blot - Anti-DARPP32 (phospho T75) antibody (AB51114)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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