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AB206695

Anti-DARS antibody

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(1 Publication)

Rabbit Polyclonal DARS antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DARS1 aa 1-100.

View Alternative Names

DARS, PIG40, DARS1, Aspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein, AspRS

2 Images
Immunoprecipitation - Anti-DARS antibody (AB206695)
  • IP

Supplier Data

Immunoprecipitation - Anti-DARS antibody (AB206695)

Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling DARS using ab206695 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated DARS, ab206695 was used at 0.4 μg/mL. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DARS antibody (ab206695)

Predicted band size: 57 kDa

false

Western blot - Anti-DARS antibody (AB206695)
  • WB

Supplier Data

Western blot - Anti-DARS antibody (AB206695)

All lanes:

Western blot - Anti-DARS antibody (ab206695) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate in NETN lysis buffer at 50 µg

Lane 2:

293T whole cell lysate in NETN lysis buffer at 50 µg

Lane 3:

Jurkat whole cell lysate in NETN lysis buffer at 50 µg

Lane 4:

TCMK whole cell lysate in NETN lysis buffer at 50 µg

Lane 5:

NIH 3T3 whole cell lysate in NETN lysis buffer at 50 µg

Predicted band size: 57 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DARS1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P14868

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction : the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
See full target information DARS1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:6685 PubMed39107301

2024

Heterozygous de novo dominant negative mutation of REXO2 results in interferonopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Elina Idiiatullina,Mahmoud Al-Azab,Meng Lin,Katja Hrovat-Schaale,Ziyang Liu,Xiaotian Li,Caiqin Guo,Xixi Chen,Yaoying Li,Song Gao,Jun Cui,Wenhao Zhou,Li Liu,Yuxia Zhang,Seth L Masters
View all publications
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Product promise

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