AB250599

Anti-DARS antibody [EPR14847(B)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal DARS antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DARS, PIG40, DARS1, Aspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein, AspRS

2 Images

Flow Cytometry (Intracellular) - Anti-DARS antibody [EPR14847(B)] - BSA and Azide free (AB250599)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DARS antibody [EPR14847(B)] - BSA and Azide free (AB250599)

This data was developed using ab182157, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of DARS expression in K562 cells using ab182157 at 1/40 dilution (red) and a rabbit IgG as negative control (green).

Supplier Data

Western blot - Anti-DARS antibody [EPR14847(B)] - BSA and Azide free (AB250599)

This data was developed using ab182157, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DARS antibody [EPR14847(B)] (<a href='/en-us/products/primary-antibodies/dars-antibody-epr14847b-ab182157'>ab182157</a>) at 1/5000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Raji cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14847(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250599 is the carrier-free version of ab182157.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction : the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

See full target information DARS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DARS antibody [EPR14847(B)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-DARS antibody [EPR14847(B)] - BSA and Azide free (AB250599)

Western blot - Anti-DARS antibody [EPR14847(B)] - BSA and Azide free (AB250599)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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