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AB184183

Anti-DAZAP1 antibody [EPR14400-60]

4

(3 Reviews)

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(1 Publication)

Rabbit Recombinant Monoclonal DAZAP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DAZ-associated protein 1, Deleted in azoospermia-associated protein 1, DAZAP1

6 Images
Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)
  • IP

Supplier Data

Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)

Western blot analysis of DAZAP1 in Jurkat cell lysate immunoprecipitated using ab184183 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of Jurkat lysate.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

Predicted band size: 43 kDa

false

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)
  • WB

Supplier Data

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)

All lanes:

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)
  • WB

Lab

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)

Lanes 1-4 : Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.

ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in CRISPR/Cas9 edited cell line ab266469 (CRISPR/Cas9 edited cell lysate ab257911) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 CRISPR/Cas9 edited samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAZAP1 CRISPR/Cas9 edited HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human DAZAP1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-dazap1-knockout-hek-293t-cell-line-ab266469'>ab266469</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)
  • WB

Lab

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)

Lanes 1-4 : Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.

ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in knockout cell line ab266470 (knockout cell lysate ab257912) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 knockout samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/20000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAZAP1 knockout HEK293T cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 43 kDa

false

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)
  • WB

Lab

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)

Lanes 1-4 : Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.

ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in CRISPR/Cas9 edited cell line ab266470 (CRISPR/Cas9 edited cell lysate ab257912) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 CRISPR/Cas9 edited samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAZAP1 CRISPR/Cas9 edited HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human DAZAP1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-dazap1-knockout-hek-293t-cell-line-ab266470'>ab266470</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)
  • WB

Lab

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (AB184183)

Lanes 1-4 : Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.

ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in knockout cell line ab266469 (knockout cell lysate ab257911) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 knockout samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAZAP1 knockout HEK293T cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

  • Carrier free

    Anti-DAZAP1 antibody [EPR14400-60] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14400-60

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein, which may be required during spermatogenesis.
See full target information DAZAP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The FEBS journal 291:4714-4731 PubMed39206622

2024

PELO regulates erythroid differentiation through interaction with MYC to upregulate KLF10.

Applications

Unspecified application

Species

Unspecified reactive species

Jinglan Hao,Guiqin Han,Xin Liang,Yongtong Ruan,Chen Huang,Naer Sa,Hang Hu,Bixi Hu,Zhongqi Li,Kai Zhang,Ping Gao,Xiaoming Dong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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