Anti-DAZAP1 antibody [EPR14400-60] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400-60] - BSA and Azide free (AB250737)

This data was developed using ab184183, the same antibody clone in a different buffer formulation.

Western blot analysis of DAZAP1 in Jurkat cell lysate immunoprecipitated using ab184183 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of Jurkat lysate.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400-60] (<a href='/en-us/products/primary-antibodies/dazap1-antibody-epr14400-60-ab184183'>ab184183</a>)

Predicted band size: 43 kDa

false

• WB

Lab

Western blot - Anti-DAZAP1 antibody [EPR14400-60] - BSA and Azide free (AB250737)

This data was developed using ab184183, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.

ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in CRISPR/Cas9 edited cell line ab266470 (CRISPR/Cas9 edited cell lysate ab257912) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 CRISPR/Cas9 edited samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (<a href='/en-us/products/primary-antibodies/dazap1-antibody-epr14400-60-ab184183'>ab184183</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAZAP1 CRISPR/Cas9 edited HEK293T cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

• WB

Supplier Data

Western blot - Anti-DAZAP1 antibody [EPR14400-60] - BSA and Azide free (AB250737)

This data was developed using ab184183, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (<a href='/en-us/products/primary-antibodies/dazap1-antibody-epr14400-60-ab184183'>ab184183</a>) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

• WB

Lab

Western blot - Anti-DAZAP1 antibody [EPR14400-60] - BSA and Azide free (AB250737)

This data was developed using ab184183, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.

ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in CRISPR/Cas9 edited cell line ab266469 (CRISPR/Cas9 edited cell lysate ab257911) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 CRISPR/Cas9 edited samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (<a href='/en-us/products/primary-antibodies/dazap1-antibody-epr14400-60-ab184183'>ab184183</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAZAP1 CRISPR/Cas9 edited HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human DAZAP1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-dazap1-knockout-hek-293t-cell-line-ab266469'>ab266469</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false