Anti-DAZAP1 antibody [EPR14400] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DAZAP1 antibody [EPR14400] - BSA and Azide free (AB250616)

This data was developed using ab182558, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling DAZAP1 with ab182558 at 1/10 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400] - BSA and Azide free (AB250616)

This data was developed using ab182558, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab182558 at 1/50 dilution (Lane 1). Lane 2 : Negative control.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400] (<a href='/en-us/products/primary-antibodies/dazap1-antibody-epr14400-ab182558'>ab182558</a>)

Predicted band size: 43 kDa

false

• WB

Supplier Data

Western blot - Anti-DAZAP1 antibody [EPR14400] - BSA and Azide free (AB250616)

This data was developed using ab182558, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DAZAP1 antibody [EPR14400] (<a href='/en-us/products/primary-antibodies/dazap1-antibody-epr14400-ab182558'>ab182558</a>) at 1/50000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false