Name: Anti-DAZL antibody [EPR21028]
SKU: ab215718
Rating: 4 (8 reviews)

Anti-DAZL antibody [EPR21028] (ab215718) is a rabbit monoclonal antibody detecting DAZL in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 10 publications

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody [EPR21028] (AB215718), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	IHC-Fr	IHC-P
Human	Expected	Expected	Tested	Expected	Tested
Mouse	Tested	Tested	Tested	Tested	Tested
Rat	Expected	Expected	Tested	Tested	Tested
Cow	Predicted	Predicted	Predicted	Predicted	Predicted
Horse	Predicted	Predicted	Predicted	Predicted	Predicted
Pig	Predicted	Predicted	Predicted	Predicted	Predicted
Sheep	Predicted	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Sheep, Horse, Cow, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Sheep, Horse, Cow, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Sheep, Horse, Cow, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/300	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/60	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Sheep, Horse, Cow, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/8000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Sheep, Horse, Cow, Pig	Dilution info -	Notes -

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

See full target information Dazl

Function

RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts.

Alternative names

Dazl1, Dazla, Dazl, Deleted in azoospermia-like, DAZ-like autosomal, Deleted in azoospermia-like 1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR21028
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-DAZL antibody [EPR21028] (ab215718) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of DAZL?

Anti-DAZL [EPR21028] (ab215718) specifically detects a band for DAZL (UniProt: Q64368) at a molecular weight of 33kDa.

Trusted by the scientific community

Anti-DAZL [EPR21028] (ab215718) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

Reviewed by scientists

Anti-DAZL [EPR21028] (ab215718) has over 5 independent reviews from customers.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-DAZL antibody [EPR21028] (ab215718) has been confirmed by Western blot testing in Dazl Knockout mouse testis samples.

Other related products

We have a range of other formats of antibody clone [EPR21028] also available for your convenience:
ab215718, Carrier free - ab228135, Alexa Fluor® 594 - ab314161, Alexa Fluor® 647 - ab316874

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody [EPR21028] (ab215718)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DAZL with ab215718 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in primary spermatocytes of human testis (PMID: 24746554) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-DAZL antibody [EPR21028] (ab215718)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen mouse testis tissue labeling DAZL with ab215718 at 1/300 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® staining in the pachytene spermatocytes and secondary spermatocytes, not present in Sertoli, Leydig or myoid cells (PMID: 24746554).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Immunoprecipitation - Anti-DAZL antibody [EPR21028] (ab215718)
DAZL was immunoprecipitated from 0.35 mg mouse testis lysate with ab215718 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215718 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.
Lane 1: Mouse testis lysate 10 μg (Input).
Lane 2: ab215718 IP in mouse testis lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab215718 in mouse testis lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-DAZL antibody [EPR21028] (ab215718)
Predicted band size: 33 kDa
This image is courtesy of an anonymous collaborator customer review.
Western blot - Anti-DAZL antibody [EPR21028] (ab215718)
10% gel.
Blocked with 5% milk for 1 hour at room temperature.
Incubated with the primary antibody for 12 hours at 4°C in TBST.
All lanes: Western blot - Anti-DAZL antibody [EPR21028] (ab215718) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 25 µg
Lane 2: DAZL knockout mouse testis tissue lysate at 25 µg
Secondary
All lanes: HRP-conjugated horse anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 33 kDa
Observed band size: 37 kDa
Exposure time: 3s
Western blot - Anti-DAZL antibody [EPR21028] (ab215718)
Blocking/Dilution buffer: 5% NFDM/TBST.
The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.
All lanes: Western blot - Anti-DAZL antibody [EPR21028] (ab215718) at 1/1000 dilution
Lane 1: Mouse testis lysate at 20 µg
Lane 2: Rat testis lysate at 20 µg
Lane 3: Human testis lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 38 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody [EPR21028] (ab215718)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling DAZL with ab215718 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in spermatogonia and primary spermatocytes of mouse testis (PMID: 24746554) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody [EPR21028] (ab215718)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling DAZL with ab215718 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in spermatogonia and primary spermatocytes of rat testis (PMID: 24746554) is observed. Counterstained with hematoxylin
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-DAZL antibody [EPR21028] (ab215718)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen rat testis tissue labeling DAZL with ab215718 at 1/60 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in the pachytene spermatocytes and secondary spermatocytes, not present in Sertoli, Leydig or myoid cells (PMID: 24746554).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-DAZL antibody [EPR21028] (ab215718)
ab215718 staining DAZL in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab215718 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-DAZL antibody [EPR21028] (ab215718)
DAZL western blot using anti-DAZL antibody [EPR21028] ab215718. Publication image and figure legend from Yang, C. R., Rajkovic, G., et al., 2020, Nat Commun, PubMed 32170089.

ab215718 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab215718 please see the product overview.
Interference with Dazl mRNA translation depletes oocytes of the DAZL protein and inhibits translation of a specific downstream target.a DAZL protein accumulation during the transition from the GV-to-MI stages of oocyte maturation. GV stage oocytes from wild type mice were cultured in vitro up to 8 h of maturation and used for Western blot analysis. 150 oocytes per lane was loaded on the gel and accumulation of α-tubulin was used as a loading control. The complete time course was performed once, while changes between the first and last time point confirmed in two additional independent experiments (see Supplementary Fig 1a). b Morpholino oligonucleotide (MO) down-regulation of DAZL protein. GV stage oocytes from RiboTagfl/fl:Zp3-Cre:WT or RiboTagfl/fl:Zp3-Cre:DAZL+/− mice were injected with CON-MO or DAZL-MO as described in the methods. After 6 h, oocytes were collected and used for Western blot analysis. A representative experiment of the three performed is reported. c–e GV stage oocytes from RiboTagfl/fl:Zp3-Cre or RiboTagfl/fl:Zp3-Cre:Dazl+/− mice were injected with CON-MO or DAZL-MO and preincubated overnight in 2 µM milrinone, then cultured in inhibitor-free medium for maturation. Oocytes were collected at 0 and 6 h for RiboTag IP followed by qPCR analysis. Ribosome loading of the endogenous Dazl mRNA (unpaired two tailed t-test, p = 0.0022) and Tex19.1 (unpaired two tailed t-test, p < 0.0001), but not CcnB1 (unpaired two tailed t-test, P = 0.8567) mRNA is disrupted after DAZL depletion. (GV: germinal vesicle; MI: Meiosis I. Each dot represents an independent biological sample collected from different experiments done in different days. f–h Input data for the immunoprecipitation reported in c–e. When N > 2, data are presented as Mean ± SEM and each dot represents an independent biological observation. Statistical significance among the different samples was evaluated with the Brown-Forsythe ANOVA test, assuming unequal SD. Calculated P values: Dazl = 0.6680, Tex19.1 = 0.9713, CcnB1 = 0.5404.