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AB50970

Anti-DB1 antibody [ZNF5J141]

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(3 Publications)

Mouse Monoclonal DB1 antibody. Suitable for ICC, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VEZF1.

View Alternative Names

DB1, ZNF161, VEZF1, Vascular endothelial zinc finger 1, Putative transcription factor DB1, Zinc finger protein 161

3 Images
Immunocytochemistry - Anti-DB1 antibody [ZNF5J141] (AB50970)
  • ICC

Unknown

Immunocytochemistry - Anti-DB1 antibody [ZNF5J141] (AB50970)

ab50970 at 1/1 dilution staining human DB1 in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat Anti-mouse IgG at 1/200 as secondary.

Western blot - Anti-DB1 antibody [ZNF5J141] (AB50970)
  • WB

Unknown

Western blot - Anti-DB1 antibody [ZNF5J141] (AB50970)

All lanes:

Western blot - Anti-DB1 antibody [ZNF5J141] (ab50970) at 1/50 dilution

All lanes:

NIH3T3 whole cell lysate at 50 µg

Secondary

All lanes:

anti-mouse IgG at 1/2500 dilution

Predicted band size: 57 kDa

Observed band size: 60 kDa

true

Western blot - Anti-DB1 antibody [ZNF5J141] (AB50970)
  • WB

Unknown

Western blot - Anti-DB1 antibody [ZNF5J141] (AB50970)

All lanes:

Western blot - Anti-DB1 antibody [ZNF5J141] (ab50970) at 1/50 dilution

All lanes:

HeLa whole cell lysate at 50 µg

Secondary

All lanes:

anti-mouse IgG at 1/2500 dilution

Predicted band size: 57 kDa

Observed band size: 60 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ZNF5J141

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human VEZF1. The exact immunogen used to generate this antibody is proprietary information.

Q14119

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/1", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab50970 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1136% Disodium hydrogenorthophosphate, 0.0225% Potassium chloride, 0.0204% Dibasic monohydrogen potassium phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Possible transcription factor. Specifically binds to the CT/GC-rich region of the interleukin-3 promoter and mediates tax transactivation of IL-3.
See full target information VEZF1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 11:1109648 PubMed36923254

2023

ETV2 and VEZF1 interaction and regulation of the hematoendothelial lineage during embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Satyabrata Das,Vinayak Gupta,Johannes Bjorge,Xiaozhong Shi,Wuming Gong,Mary G Garry,Daniel J Garry

Journal of vascular research 56:97-106 PubMed31030195

2019

Inhibition of miR-495 Improves Both Vascular Remodeling and Angiogenesis in Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Fu,Peiyuan Bai,Yiwei Chen,Tingting Yu,Fen Li

Nature communications 4:2824 PubMed24280686

2013

RhoB controls coordination of adult angiogenesis and lymphangiogenesis following injury by regulating VEZF1-mediated transcription.

Applications

WB

Species

Unspecified reactive species

Damien Gerald,Irit Adini,Sharon Shechter,Carole Perruzzi,Joseph Varnau,Benjamin Hopkins,Shiva Kazerounian,Peter Kurschat,Stephanie Blachon,Santosh Khedkar,Mandrita Bagchi,David Sherris,George C Prendergast,Michael Klagsbrun,Heidi Stuhlmann,Alan C Rigby,Janice A Nagy,Laura E Benjamin
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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