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AB235528

Anti-DBF4B/DRF1 antibody

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(1 Publication)

Rabbit Polyclonal DBF4B/DRF1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DBF4B aa 1-300.

View Alternative Names

ASKL1, DRF1, DBF4B, Protein DBF4 homolog B, Activator of S phase kinase-like protein 1, Chiffon homolog B, Dbf4-related factor 1, ASK-like protein 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBF4B/DRF1 antibody (AB235528)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBF4B/DRF1 antibody (AB235528)

Paraffin-embedded human testis tissue stained for DBF4B/DRF1 using ab235528 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-DBF4B/DRF1 antibody (AB235528)
  • WB

Supplier Data

Western blot - Anti-DBF4B/DRF1 antibody (AB235528)

All lanes:

Western blot - Anti-DBF4B/DRF1 antibody (ab235528) at 1/500 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 2:

A549 (human lung carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DBF4B aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q8NFT6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.
See full target information DBF4B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular oncology (Dordrecht) 46:1493-1507 PubMed37219768

2023

TRIM17-mediated ubiquitination and degradation of RBM38 promotes cisplatin resistance in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Zhong,Jing Zhang,Xingren Liu,Hongmin Li
View all publications
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