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AB236398

Anti-DBL antibody [OTI2C12]

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(1 Publication)

Mouse Monoclonal DBL antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MCF2 aa 850 to C-terminus.

View Alternative Names

DBL, MCF2, Proto-oncogene DBL, Proto-oncogene MCF-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBL antibody [OTI2C12] (AB236398)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBL antibody [OTI2C12] (AB236398)

Paraffin-embedded human embryonic cerebellum tissue stained for DBL using ab236398 at 1/500 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.

Western blot - Anti-DBL antibody [OTI2C12] (AB236398)
  • WB

Supplier Data

Western blot - Anti-DBL antibody [OTI2C12] (AB236398)

All lanes:

Western blot - Anti-DBL antibody [OTI2C12] (ab236398) at 1/500 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY DBL cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Predicted band size: 108 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2C12

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MCF2 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P10911

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.
See full target information MCF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:3807-3825 PubMed37166429

2023

Identification and validation of metabolism-related genes signature and immune infiltration landscape of rheumatoid arthritis based on machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoyang Guo,Yuanye Ma,Yaqing Wang,Hongfei Xiang,Huifei Cui,Zuoran Fan,Youfu Zhu,Dongming Xing,Bohua Chen,Hao Tao,Zhu Guo,Xiaolin Wu
View all publications
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