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AB154230

Anti-DBR1 antibody

5

(2 Reviews)

|

(1 Publication)

Rabbit Polyclonal DBR1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DBR1 aa 50-350.

View Alternative Names

Lariat debranching enzyme, DBR1

2 Images
Western blot - Anti-DBR1 antibody (AB154230)
  • WB

Unknown

Western blot - Anti-DBR1 antibody (AB154230)

7.5% SDS PAGE

All lanes:

Western blot - Anti-DBR1 antibody (ab154230) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 62 kDa

false

Western blot - Anti-DBR1 antibody (AB154230)
  • WB

AbReview40872****

Western blot - Anti-DBR1 antibody (AB154230)

All lanes:

Western blot - Anti-DBR1 antibody (ab154230) at 1/10000 dilution

Lane 1:

Xenopus tropicalis oocyte 5 GV (nuclear) lysate at 5 Cells

Lane 2:

Xenopus tropicalis oocyte cytoplamsic lysate at 5 Cells

Secondary

All lanes:

HRP-conjugated anti-rabbit polyclonal at 1/20000 dilution

Predicted band size: 62 kDa

Observed band size: 53 kDa

true

Exposure time: 10s

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human DBR1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q9UK59

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Cleaves the 2'-5' phosphodiester linkage at the branch point of excised lariat intron RNA and converts them into linear molecules that can be subsequently degraded, thereby facilitating ribonucleotide turnover (PubMed : 10982890, PubMed : 16232320, PubMed : 2435736). Linked to its role in pre-mRNA processing mechanism, may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis and have an antiviral cell-intrinsic defense function in the brainstem (PubMed : 16232320, PubMed : 29474921).
See full target information DBR1

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

RNA (New York, N.Y.) 20:1476-87 PubMed25051970

2014

Lariat intronic RNAs in the cytoplasm of Xenopus tropicalis oocytes.

Applications

Unspecified application, Unspecified application

Species

Xenopus laevis, Xenopus tropicalis

Gaëlle J S Talhouarne,Joseph G Gall
View all publications

Product promise

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