Rabbit Polyclonal DBR1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DBR1 aa 50-350.
Images
Publications
- RNA (New York, N.Y.) 20:1476-872014Lariat intronic RNAs in the cytoplasm of Xenopus tropicalis oocytes.Applications:Unspecified applicationReactive species:Xenopus laevis, Xenopus tropicalisGaëlle J S Talhouarne et. al.PubMed 25051970
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DBR1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UK59
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis.
Alternative names
Lariat debranching enzyme, DBR1
Recommended products
Rabbit Polyclonal DBR1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DBR1 aa 50-350.
Alternative names
Lariat debranching enzyme, DBR1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DBR1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UK59
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
This image is courtesy of an anonymous customer reviewWestern blot - Anti-DBR1 antibody (ab154230)
All lanes: Western blot - Anti-DBR1 antibody (ab154230) at 1/10000 dilution
Lane 1: Xenopus tropicalis oocyte 5 GV (nuclear) lysate at 5 Cells
Lane 2: Xenopus tropicalis oocyte cytoplamsic lysate at 5 Cells
SecondaryAll lanes: HRP-conjugated anti-rabbit polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 53 kDa
Exposure time: 10s
Western blot - Anti-DBR1 antibody (ab154230)
7.5% SDS PAGE
All lanes: Western blot - Anti-DBR1 antibody (ab154230) at 1/1000 dilution
All lanes: A549 whole cell lysate at 30 µg
Predicted band size: 62 kDa
Downloads
Product protocols
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