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AB151991

Anti-DBT antibody

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(6 Publications)

Rabbit Polyclonal DBT antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DBT aa 1-250.

View Alternative Names

BCATE2, BCKDHE2, DBT, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis, Branched chain 2-oxo-acid dehydrogenase complex component E2, Branched-chain alpha-keto acid dehydrogenase complex component E2, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, Dihydrolipoamide branched chain transacylase, Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, BCOADC-E2, BCKAD-E2, BCKADE2, BCKDH-E2

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-DBT antibody (AB151991)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DBT antibody (AB151991)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling DBT protein at mitochondria using ab151991 at a 1/500 dilution (Green).
Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm

Western blot - Anti-DBT antibody (AB151991)
  • WB

Unknown

Western blot - Anti-DBT antibody (AB151991)

10% SDS PAGE

All lanes:

Western blot - Anti-DBT antibody (ab151991) at 1/1000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 53 kDa

false

Western blot - Anti-DBT antibody (AB151991)
  • WB

Supplier Data

Western blot - Anti-DBT antibody (AB151991)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-DBT antibody (ab151991) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

Western blot - Anti-DBT antibody (AB151991)
  • WB

Supplier Data

Western blot - Anti-DBT antibody (AB151991)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-DBT antibody (ab151991) at 1/1000 dilution

All lanes:

rat liver lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

Western blot - Anti-DBT antibody (AB151991)
  • WB

Supplier Data

Western blot - Anti-DBT antibody (AB151991)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-DBT antibody (ab151991) at 1/1000 dilution

All lanes:

mouse liver lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human DBT aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P11182

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components : branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.
See full target information DBT

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed39255192

2024

DBT is a metabolic switch for maintenance of proteostasis under proteasomal impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Ran-Der Hwang,YuNing Lu,Qing Tang,Goran Periz,Giho Park,Xiangning Li,Qiwang Xiang,Yang Liu,Tao Zhang,Jiou Wang

Scientific reports 14:8013 PubMed38580754

2024

Proteomic analysis of DEN and CCl-induced hepatocellular carcinoma mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Yuhui Liu,Liangliang Ren,Junqing Li,Weiran Lin,Lijuan Lou,Minghan Wang,Chaoying Li,Ying Jiang

The Journal of biological chemistry 299:105333 PubMed37827290

2023

Reactive nitrogen species inhibit branched chain alpha-ketoacid dehydrogenase complex and impact muscle cell metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas L Arp,Gretchen L Seim,James A Votava,Jordyn Josephson,Jing Fan

Physiological reports 11:e15608 PubMed36802195

2023

Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Toshifumi Ogawa,Hidemichi Kouzu,Arata Osanami,Yuki Tatekoshi,Tatsuya Sato,Atsushi Kuno,Yugo Fujita,Shoya Ino,Masaki Shimizu,Yuki Toda,Wataru Ohwada,Toshiyuki Yano,Masaya Tanno,Takayuki Miki,Tetsuji Miura

Cell stem cell 29:1346-1365.e10 PubMed36055191

2022

Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Thi Xuan Ai Pham,Amitesh Panda,Harunobu Kagawa,San Kit To,Cankat Ertekin,Grigorios Georgolopoulos,Sam S F A van Knippenberg,Ryan Nicolaas Allsop,Alexandre Bruneau,Jonathan Sai-Hong Chui,Lotte Vanheer,Adrian Janiszewski,Joel Chappell,Michael Oberhuemer,Raissa Songwa Tchinda,Irene Talon,Sherif Khodeer,Janet Rossant,Frederic Lluis,Laurent David,Nicolas Rivron,Bradley Philip Balaton,Vincent Pasque

Nature metabolism 2:775-792 PubMed32694827

2020

Tumour-reprogrammed stromal BCAT1 fuels branched-chain ketoacid dependency in stromal-rich PDAC tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwen Zhu,Abhinav Achreja,Noah Meurs,Olamide Animasahun,Sarah Owen,Anjali Mittal,Pooja Parikh,Ting-Wen Lo,Janusz Franco-Barraza,Jiaqi Shi,Valerie Gunchick,Mara H Sherman,Edna Cukierman,Andrew M Pickering,Anirban Maitra,Vaibhav Sahai,Meredith A Morgan,Sunitha Nagrath,Theodore S Lawrence,Deepak Nagrath
View all publications

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