Rabbit Polyclonal DBT antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DBT aa 1-250.
View Alternative Names
BCATE2, BCKDHE2, DBT, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis, Branched chain 2-oxo-acid dehydrogenase complex component E2, Branched-chain alpha-keto acid dehydrogenase complex component E2, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, Dihydrolipoamide branched chain transacylase, Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, BCOADC-E2, BCKAD-E2, BCKADE2, BCKDH-E2
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-DBT antibody (AB151991)
Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling DBT protein at mitochondria using ab151991 at a 1/500 dilution (Green).
Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm
- WB
Unknown
Western blot - Anti-DBT antibody (AB151991)
10% SDS PAGE
All lanes:
Western blot - Anti-DBT antibody (ab151991) at 1/1000 dilution
Lane 1:
H1299 whole cell lysate at 30 µg
Lane 2:
HepG2 whole cell lysate at 30 µg
Predicted band size: 53 kDa
false
- WB
Supplier Data
Western blot - Anti-DBT antibody (AB151991)
Samples were separated by 10% SDS-PAGE.
All lanes:
Western blot - Anti-DBT antibody (ab151991) at 1/1000 dilution
Lane 1:
293T whole cell lysates at 30 µg
Lane 2:
A431 whole cell lysates at 30 µg
Lane 3:
HeLa whole cell lysates at 30 µg
Lane 4:
HepG2 whole cell lysates at 30 µg
Secondary
All lanes:
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 54 kDa
false
- WB
Supplier Data
Western blot - Anti-DBT antibody (AB151991)
Samples were separated by 10% SDS-PAGE.
All lanes:
Western blot - Anti-DBT antibody (ab151991) at 1/1000 dilution
All lanes:
rat liver lysate at 50 µg
Secondary
All lanes:
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 54 kDa
false
- WB
Supplier Data
Western blot - Anti-DBT antibody (AB151991)
Samples were separated by 10% SDS-PAGE.
All lanes:
Western blot - Anti-DBT antibody (ab151991) at 1/1000 dilution
All lanes:
mouse liver lysate at 50 µg
Secondary
All lanes:
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 54 kDa
false
Reactivity data
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (6)
Recent publications for all applications. Explore the full list and refine your search
eLife 12: PubMed39255192
2024
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 14:8013 PubMed38580754
2024
Applications
Unspecified application
Species
Unspecified reactive species
The Journal of biological chemistry 299:105333 PubMed37827290
2023
Applications
Unspecified application
Species
Unspecified reactive species
Physiological reports 11:e15608 PubMed36802195
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cell stem cell 29:1346-1365.e10 PubMed36055191
2022
Applications
Unspecified application
Species
Unspecified reactive species
Nature metabolism 2:775-792 PubMed32694827
2020
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com