AB5715

Anti-DC-SIGN antibody

Name: Anti-DC-SIGN antibody (ab5715) | Abcam
Brand: Abcam Limited
SKU: AB5715

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(4 Publications)

Rabbit Polyclonal DC-SIGN antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CD209 aa 350 to C-terminus.

View Alternative Names

CD209, CLEC4L, CD209 antigen, C-type lectin domain family 4 member L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, DC-SIGN, DC-SIGN1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody (AB5715)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody (AB5715)

Immunohistochemistry of DC-SIGN in human lymph node tissue with DC-SIGN antibody at 10 µg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody (AB5715)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody (AB5715)

Immunofluorescence of DC-SIGN in Human Lymph Node cells using ab5715 at 20 ug/ml.

Unknown

Western blot - Anti-DC-SIGN antibody (AB5715)

ab5715 at 0.5 μg /ml staining approximately 44kDa of human CD209 in Human placenta lysate by Western blot (ECL).

ab5715 at 0.5 µg /ml staining approximately 44kDa of human CD209 in Human placenta lysate by Western blot (ECL).

All lanes:

Western blot - Anti-DC-SIGN antibody (ab5715)

Predicted band size: 46 kDa

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Western blot - Anti-DC-SIGN antibody (AB5715)

Lane 1:

Western blot - Anti-DC-SIGN antibody (ab5715) at 1 µg/mL

Lane 2:

Western blot - Anti-DC-SIGN antibody (ab5715) at 2 µg/mL

All lanes:

Human placenta

Predicted band size: 46 kDa

Observed band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CD209 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NNX6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response.. On dendritic cells (DCs) it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates (PubMed : 10721994, PubMed : 10721995, PubMed : 11017109, PubMed : 12574325). May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2 (PubMed : 11017109). Forms a first contact between DC and resting T cell, througth ICAM3 binding, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells (PubMed : 10721994, PubMed : 10721995).. (Microbial infection) Acts as an attachment receptor for HIV-1 and HIV-2.. (Microbial infection) Acts as an attachment receptor for Ebolavirus.. (Microbial infection) Acts as an attachment receptor for Cytomegalovirus.. (Microbial infection) Acts as an attachment receptor for HCV.. (Microbial infection) Acts as an attachment receptor for Dengue virus.. (Microbial infection) Acts as an attachment receptor for Measles virus.. (Microbial infection) Acts as an attachment receptor for Herpes simplex virus 1.. (Microbial infection) Acts as an attachment receptor for Influenzavirus A.. (Microbial infection) Acts as an attachment receptor for SARS-CoV.. (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus.. (Microbial infection) Acts as an attachment receptor for Lassa virus (PubMed : 23966408). Acts as an attachment receptor for Marburg virusn.. (Microbial infection) Acts as an attachment receptor for Respiratory syncytial virus.. (Microbial infection) Acts as an attachment receptor for Rift valley fever virus and uukuniemi virus.. (Microbial infection) Acts as an attachment receptor for West-nile virus.. (Microbial infection) Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of bacterial pathogen antigens, including Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA (PubMed : 16379498). Recognition of M.tuberculosis by dendritic cells occurs partially via this molecule (PubMed : 16092920, PubMed : 21203928).

See full target information CD209

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Reproductive biology and endocrinology : RB&E 20:48 PubMed35260161

2022

CSF-1-induced DC-SIGN macrophages are present in the ovarian endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Xiaocui,Hong Wei,Cai Yunlang,Zheng Zhenzhen,An Min

NPJ biofilms and microbiomes 7:87 PubMed34880222

2021

Carbohydrates from Pseudomonas aeruginosa biofilms interact with immune C-type lectins and interfere with their receptor function.

Applications

Unspecified application

Species

Unspecified reactive species

Sonali Singh,Yasir Almuhanna,Mohammad Y Alshahrani,Douglas W Lowman,Peter J Rice,Chris Gell,Zuchao Ma,Bridget Graves,Darryl Jackson,Kelly Lee,Rucha Juarez,Janice Koranteng,Sirina Muntaka, Daniel A Mitchell,Ana C da Silva,Farah Hussain,Gokhan Yilmaz,Francesca Mastrotto,Yasuhiko Irie,Paul Williams,David L Williams,Miguel Cámara,Luisa Martinez-Pomares

Nature communications 11:2759 PubMed32488028

2020

Human norovirus targets enteroendocrine epithelial cells in the small intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Kim Y Green,Stuart S Kaufman,Bianca M Nagata,Natthawan Chaimongkol,Daniel Y Kim,Eric A Levenson,Christine M Tin,Allison Behrle Yardley,Jordan A Johnson,Ana Beatriz F Barletta,Khalid M Khan,Nada A Yazigi,Sukanya Subramanian,Sangeetha R Moturi,Thomas M Fishbein,Ian N Moore,Stanislav V Sosnovtsev

Science translational medicine 6:229ra40 PubMed24670684

2014

Defective neutrophil recruitment in leukocyte adhesion deficiency type I disease causes local IL-17-driven inflammatory bone loss.

Applications

Unspecified application

Species

Unspecified reactive species

Niki M Moutsopoulos,Joanne Konkel,Mojgan Sarmadi,Mehmet A Eskan,Teresa Wild,Nicolas Dutzan,Loreto Abusleme,Camille Zenobia,Kavita B Hosur,Toshiharu Abe,Gulbu Uzel,Wanjun Chen,Triantafyllos Chavakis,Steven M Holland,George Hajishengallis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DC-SIGN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody (AB5715)

Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody (AB5715)

Western blot - Anti-DC-SIGN antibody (AB5715)

Western blot - Anti-DC-SIGN antibody (AB5715)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (4)

Product promise