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AB245115

Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72]

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(1 Publication)

Rabbit Recombinant Monoclonal DC-SIGN antibody. Suitable for IP, Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD209, CLEC4L, CD209 antigen, C-type lectin domain family 4 member L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, DC-SIGN, DC-SIGN1, CD299, CD209L, CD209L1, CLEC4M, C-type lectin domain family 4 member M, CD209 antigen-like protein 1, DC-SIGN-related protein, Dendritic cell-specific ICAM-3-grabbing non-integrin 2, Liver/lymph node-specific ICAM-3-grabbing non-integrin, DC-SIGNR, DC-SIGN2, L-SIGN

8 Images
Flow Cytometry - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)

Flow cytometric analysis of unfixed non-permeabilized THP-1 (human monocytic leukemia cell line) cells treated with 10 ng/ml PMA for 18 hours, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN+DC-SIGNR was stained in treated (red) and untreated (green) cells using ab245115 at 1/600 dilution as compared to a Rabbit monoclonal IgG (ab172730, black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody / blue). The secondary antibody was a Goat anti rabbit IgG (Dylight® 488, ab98462) used at 1/2000 dilution. Gated on viable cells. DC-SIGN expression is induced by PMA plus IL4 in THP-1 (PMID : 15070901; PMID : 22675249). DC-SIGNR/CD299 expression is induced by PMA in THP-1 (PMID 30077333).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling DC-SIGN + DC-SIGNR with ab245115 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining on sinusoidal endothelial cells of human liver (PMID : 16816373) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling DC-SIGN + DC-SIGNR with ab245115 at 1/100 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) seconday antibody (green) at 1/1000 dilution.

Confocal image showing cytoplasmic and membranous staining in THP-1 cells treated with 10 ng/ml PMA for 18 hours, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN expression is induced by PMA plus IL4 in THP-1(PMID : 15070901; PMID : 22675249). DC-SIGNR/CD299 expression is induced by PMA in THP-1 (PMID 30077333). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889)(red) at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling DC-SIGN + DC-SIGNR with ab245115 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining on dendritic cell-like cells of human skin (PMID : 11859097; PMID : 11859097) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling DC-SIGN + DC-SIGNR with ab245115 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining on dendritic cell-like cells of human tonsil (PMID : 11859097) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)
  • IP

Unknown

Immunoprecipitation - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)

DC-SIGN+DC-SIGNR was immunoprecipitated from 0.35 mg human tonsil tissue lysate using ab245115 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab245115 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

Lane 1 : Human tonsil tissue lysate 10 μg (input).
Lane 2 : ab245115 IP in Human tonsil tissue.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab245115 in Human tonsil lysate. (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

Observed band size: 44 kDa

false

Western blot - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)
  • WB

Unknown

Western blot - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)

Exposure time :

Lane 1 : 3 seconds;

Lane 2 : 92 seconds;

Lane 3 : 37 seconds

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115) at 1/1000 dilution

Lane 1:

Human lymph node tissue lysate at 20 µg

Lane 2:

Human skin tissue lysate at 20 µg

Lane 3:

Human thymus tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 46 kDa

false

Western blot - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)
  • WB

Unknown

Western blot - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (AB245115)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115) at 1/1000 dilution

Lane 1:

Recombinant Human DC-SIGN/CD209 Protein (aa59-404) 20 ng

Lane 2:

Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein (aa73-376) 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 48 kDa,66 kDa

false

Exposure time: 3s

  • Carrier free

    Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22395-72

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, ICC/IF, WB, Flow Cyt, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] - BSA and Azide free (AB240156)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/600", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response.. On dendritic cells (DCs) it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates (PubMed : 10721994, PubMed : 10721995, PubMed : 11017109, PubMed : 12574325). May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2 (PubMed : 11017109). Forms a first contact between DC and resting T cell, througth ICAM3 binding, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells (PubMed : 10721994, PubMed : 10721995).. (Microbial infection) Acts as an attachment receptor for HIV-1 and HIV-2.. (Microbial infection) Acts as an attachment receptor for Ebolavirus.. (Microbial infection) Acts as an attachment receptor for Cytomegalovirus.. (Microbial infection) Acts as an attachment receptor for HCV.. (Microbial infection) Acts as an attachment receptor for Dengue virus.. (Microbial infection) Acts as an attachment receptor for Measles virus.. (Microbial infection) Acts as an attachment receptor for Herpes simplex virus 1.. (Microbial infection) Acts as an attachment receptor for Influenzavirus A.. (Microbial infection) Acts as an attachment receptor for SARS-CoV.. (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus.. (Microbial infection) Acts as an attachment receptor for Lassa virus (PubMed : 23966408). Acts as an attachment receptor for Marburg virusn.. (Microbial infection) Acts as an attachment receptor for Respiratory syncytial virus.. (Microbial infection) Acts as an attachment receptor for Rift valley fever virus and uukuniemi virus.. (Microbial infection) Acts as an attachment receptor for West-nile virus.. (Microbial infection) Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of bacterial pathogen antigens, including Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA (PubMed : 16379498). Recognition of M.tuberculosis by dendritic cells occurs partially via this molecule (PubMed : 16092920, PubMed : 21203928).
See full target information CD209

Additional targets

CLEC4M
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:327 PubMed39747903

2025

FN1 shapes the behavior of papillary thyroid carcinoma through alternative splicing of EDB region.

Applications

Unspecified application

Species

Unspecified reactive species

Mian Liu,Pei Chen,Bo Wei,Hai-Long Tan,Ya-Xin Zhao,Lei Ai,Ning Li,Ying-Ke Jiang,Jing Lin,Shi-Jin Li,Shi Chang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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