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Anti-DCAMKL1 antibody ab31704 is a rabbit polyclonal antibody that is used in DCAMKL1 western blotting and immunofluorescence. Suitable for human, mouse and rat samples.

- Specificity confirmed with DCLK1 knockout cell line validation


Images

Immunocytochemistry/ Immunofluorescence - Anti-DCAMKL1 antibody (AB31704), expandable thumbnail
  • Immunoprecipitation - Anti-DCAMKL1 antibody (AB31704), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-DCAMKL1 antibody (AB31704), expandable thumbnail
  • Western blot - Anti-DCAMKL1 antibody (AB31704), expandable thumbnail
  • Western blot - Anti-DCAMKL1 antibody (AB31704), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWB
Human
Predicted
Predicted
Tested
Mouse
Predicted
Expected
Expected
Rat
Expected
Predicted
Expected

Expected
Expected

Species

Rat

Dilution info

5 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.5 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

0.5 µg/mL

Notes

-

Species

Rat

Dilution info

0.5 µg/mL

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system (By similarity).

Alternative names

Recommended products

Anti-DCAMKL1 antibody ab31704 is a rabbit polyclonal antibody that is used in DCAMKL1 western blotting and immunofluorescence. Suitable for human, mouse and rat samples.

- Specificity confirmed with DCLK1 knockout cell line validation

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Specificity

This antibody recognizes 2 different human isoforms (AL: 82.2 KDa and BL: 47.6 KDa). From May 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab109029.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-DCAMKL1 antibody (ab31704), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DCAMKL1 antibody (ab31704)

    ab31704 staining DCAMKL1 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab31704 at 5 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunoprecipitation - Anti-DCAMKL1 antibody (ab31704), expandable thumbnail

    Immunoprecipitation - Anti-DCAMKL1 antibody (ab31704)

    DCAMKL1 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to DCAMKL1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31704.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
    Band: 82kDa: DCAMKL1; non specific - 52 and 27kDa: We are unsure as to the identity of this extra band.

    All lanes: Immunoprecipitation - Anti-DCAMKL1 antibody (ab31704)

    Predicted band size: 82 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DCAMKL1 antibody (ab31704), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DCAMKL1 antibody (ab31704)

    ICC/IF image of ab31704 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31704, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

  • Western blot - Anti-DCAMKL1 antibody (ab31704), expandable thumbnail

    Western blot - Anti-DCAMKL1 antibody (ab31704)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: DCAMKL1 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: NIH3T3 whole cell lysate (20 μg)
    Lane 4: Human brain whole tissue lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab31704 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab31704 was shown to specifically react with DCAMKL1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when DCAMKL1 knockout cells were examined. Wild-type and DCAMKL1 knockout samples were subjected to SDS-PAGE. ab31704 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-DCAMKL1 antibody (ab31704)

    Predicted band size: 82 kDa

  • Western blot - Anti-DCAMKL1 antibody (ab31704), expandable thumbnail

    Western blot - Anti-DCAMKL1 antibody (ab31704)

    The 82 kDa and 47 kDa bands correspond to the AL and BL isoforms respectively. This antibody should not detect the AS and BS isoforms of DCAMLK1.

    All lanes: Western blot - Anti-DCAMKL1 antibody (ab31704) at 1 µg/mL

    All lanes: Mouse Brain Whole Tissue Lysate at 20 µg

    Secondary

    All lanes: IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 82 kDa

    Observed band size: 47 kDa, 82 kDa

  • Western blot - Anti-DCAMKL1 antibody (ab31704), expandable thumbnail

    Western blot - Anti-DCAMKL1 antibody (ab31704)

    All lanes: Western blot - Anti-DCAMKL1 antibody (ab31704) at 1 µg/mL

    All lanes: Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 82 kDa

    Observed band size: 47 kDa, 82 kDa

  • Western blot - Anti-DCAMKL1 antibody (ab31704), expandable thumbnail

    Western blot - Anti-DCAMKL1 antibody (ab31704)

    The 82 kDa and 47 kDa bands correspond to the AL and BL isoforms respectively. DCAMLK1 has a number of potential phosphorylation sites which may explain the higher migrating bands at 52 and 54 kDa.

    All lanes: Western blot - Anti-DCAMKL1 antibody (ab31704) at 1 µg/mL

    All lanes: Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 82 kDa

    Observed band size: 30 kDa, 47 kDa, 52 kDa, 54 kDa, 82 kDa

    Exposure time: 5min

  • Western blot - Anti-DCAMKL1 antibody (ab31704), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-DCAMKL1 antibody (ab31704)

    All lanes: Western blot - Anti-DCAMKL1 antibody (ab31704) at 1 µg/mL

    Lane 1: HCT116 WT at 30 µg

    Lane 2: MiaPaCa2, at 30 µg

    Lane 3: SW480 at 30 µg

    Lane 4: SW620 at 30 µg

    Lane 5:  HT-29 at 30 µg

    Lane 6: H4 at 30 µg

    Lane 7: mouse brain lysate at 30 µg

    Secondary

    All lanes: IRDye® 800CW Goat Anti-Rabbit, at 1/20000 dilution

    Performed under reducing conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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