Anti-DCAMKL1 antibody [EPR6085]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (AB109029)

Immunohistochemical analysis of Paraffin-embedded sections rat colon tissue labelling DCAMKL1 with ab109029 at 1/4000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on rat colon tissue is observed. Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (AB109029)

Immunohistochemical analysis of Paraffin-embedded sections mouse stomach tissue labelling DCAMKL1 with ab109029 at 1/4000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on mouse stomach tissue is observed. Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• WB

Lab

Western blot - Anti-DCAMKL1 antibody [EPR6085] (AB109029)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The antibody recognizes isoforms of AL, AS, BS and BL.

All lanes:

Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029) at 1/5000 dilution

All lanes:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 82 kDa

Observed band size: 82 kDa,47 kDa

false

• WB

Lab

Western blot - Anti-DCAMKL1 antibody [EPR6085] (AB109029)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as GAPDH loading control. This antibody recognizes isoforms of AL, AS, BS and BL.

All lanes:

Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029) at 1/5000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Lane 3:

Mouse cerebllum lysate at 20 µg

Lane 4:

Rat cerebllum lysate at 20 µg

Lane 5:

Mouse colon lysate at 20 µg

Lane 6:

Rat colon lysate at 20 µg

Lane 7:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 82 kDa

Observed band size: 82 kDa,47 kDa

false

• WB

Lab

Western blot - Anti-DCAMKL1 antibody [EPR6085] (AB109029)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : Knockout HAP1 whole cell lysate (20 μg)
Lane 3 : DCLK1 (KO) whole cell lysate (20 μg)
Lane 4 : Empty whole cell lysate (20 μg)
Lane 5 : Hu brain whole cell lysate (20 μg)
Lanes 1 - 5 : Merged signal (red and green). Green - ab109029 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109029 was shown to specifically react with DCLK1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when knockout samples were used. Wild-type and knockout samples were subjected to SDS-PAGE. ab109029 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)

Predicted band size: 82 kDa

false