Anti-DCAMKL1 antibody [EPR6085] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] - BSA and Azide free (AB187184)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109029). Immunohistochemical analysis of Paraffin-embedded sections rat colon tissue labelling DCAMKL1 with ab109029 at 1/4000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on rat colon tissue is observed. Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] - BSA and Azide free (AB187184)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109029). Immunohistochemical analysis of Paraffin-embedded sections mouse stomach tissue labelling DCAMKL1 with ab109029 at 1/4000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on mouse stomach tissue is observed. Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• WB

Lab

Western blot - Anti-DCAMKL1 antibody [EPR6085] - BSA and Azide free (AB187184)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109029). Blocking and diluting buffer and concentration : 5% NFDM/TBST. The antibody recognizes isoforms of AL, AS, BS and BL.

All lanes:

Western blot - Anti-DCAMKL1 antibody [EPR6085] (<a href='/en-us/products/primary-antibodies/dcamkl1-antibody-epr6085-ab109029'>ab109029</a>) at 1/5000 dilution

All lanes:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 82 kDa

Observed band size: 82 kDa,47 kDa

false

• WB

Lab

Western blot - Anti-DCAMKL1 antibody [EPR6085] - BSA and Azide free (AB187184)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109029). Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as GAPDH loading control. This antibody recognizes isoforms of AL, AS, BS and BL.

All lanes:

Western blot - Anti-DCAMKL1 antibody [EPR6085] (<a href='/en-us/products/primary-antibodies/dcamkl1-antibody-epr6085-ab109029'>ab109029</a>) at 1/5000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Lane 3:

Mouse cerebllum lysate at 20 µg

Lane 4:

Rat cerebllum lysate at 20 µg

Lane 5:

Mouse colon lysate at 20 µg

Lane 6:

Rat colon lysate at 20 µg

Lane 7:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 82 kDa

Observed band size: 82 kDa,47 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] - BSA and Azide free (AB187184)

Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109029 at a dilution of 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109029).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.