JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB322504

Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal DCD antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

AIDD, DSEP, DCD, Dermcidin, Preproteolysin

3 Images
Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)

Sandwich ELISA of ab322513 using human DCD immunogen protein standard curve with the capture antibody ab322503 at 2 μg/mL and detector antibody ab322504 at 0.5 μg/mL. Background-subtracted absorbance data values (mean +/- standard deviation) are displayed.

Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)

Interpolated concentration of DCD was measured in duplicate at different sample concentrations. Undiluted sample is a 1 : 4 dilution from neat serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)

Interpolated concentration of DCD was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Skin tissue extract 3.9 µg/ml and cerebellum tissue extract 3.9 µg/ml. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). In the cerebellum tissue extract the signal was not detectable (ND). Data represented here are indicative of assay working using example of biological samples. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25718-6

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Product details

ab322504 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human DCD. The recommended pair for sandwich ELISA is:
Capture: ab322503, Human DCD Capture Antibody (unconjugated)
Detector: ab322504, Human DCD Detector Antibody (unconjugated)
The reference range value is 4.8-156 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

DCD-1. Found in sweat, has an antimicrobial activity during early bacterial colonization (PubMed : 11694882, PubMed : 23426625). The secreted peptide assembles into homohexameric complexes that can associate with and also insert into pathogen membranes (PubMed : 23426625). Once inserted in bacteria membranes forms anion channels probably altering the transmembrane potential essential for bacterial survival (PubMed : 23426625). Highly effective against E.coli, E.faecalis, S.aureus and C.albicans (PubMed : 11694882). Optimal pH and salt concentration resemble the conditions in sweat (PubMed : 11694882). Also exhibits proteolytic activity, cleaving on the C-terminal side of Arg and, to a lesser extent, Lys residues (PubMed : 17448443).. Survival-promoting peptide. Promotes survival of neurons and displays phosphatase activity (PubMed : 9736629). It may bind IgG (PubMed : 9736629).
See full target information DCD
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com