Anti-DCK antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DCK antibody (AB151966)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling DCK protein at nucleus using ab151966 at a 1/500 dilution (Green). Phalloidin, a cytoskeleton marker, was stained at a 1/100 dilution (Red).
Scale bar = 10 µM

• IP

Supplier Data

Immunoprecipitation - Anti-DCK antibody (AB151966)

Immunoprecipitation of DCK protein from 293T whole cell extracts using 5 ug of ab151966.
Western blot analysis was performed using ab151966.
Anti-Rabbit IgG was used as a secondary reagent.

All lanes:

Immunoprecipitation - Anti-DCK antibody (ab151966) at 5 µg

Lane 1:

293T whole cell extracts with control IgG

Lane 2:

293T whole cell extracts

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 30 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody (AB151966)

Immunohistochemical analysis of paraffin-embedded mouse hind brain tissue stained for DCK protein at nucleus on mouse hind brain with ab151966 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody (AB151966)

Immunohistochemical analysis of paraffin-embedded rat lung tissue stained for DCK protein at nucleus with ab151966 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• WB

Supplier Data

Western blot - Anti-DCK antibody (AB151966)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-DCK antibody (ab151966) at 1/5000 dilution

Lane 1:

A549 whole cell extracts at 30 µg

Lane 2:

H1299 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 30 kDa

false

• WB

Supplier Data

Western blot - Anti-DCK antibody (AB151966)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-DCK antibody (ab151966) at 1/1000 dilution

Lane 1:

PC-12 whole cell lysate at 30 µg

Lane 2:

Rat2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 30 kDa

false

• WB

Supplier Data

Western blot - Anti-DCK antibody (AB151966)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-DCK antibody (ab151966) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 30 kDa

false