Rabbit Polyclonal DCK antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DCK aa 1 to C-terminus.
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Publications
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- Acta haematologica 144:534-5412021Deoxycytidine Kinase (DCK) Mutations in Human Acute Myeloid Leukemia Resistant to Cytarabine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBiao Wu,Zhengwei Jenny Mao,Zhi Wang,Pin Wu,Hongyu Huang,Wenjun Zhao,Lei Zhang,Zhou Zhang,Hongchao Yin,Robert Peter Gale,Bin YinPubMed 33626530
- Oncology letters 20:2472020Gemcitabine resistance in triple-negative breast cancer cells can be reverted by deoxyribonucleoside kinase in the nucleus or cytosol.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zhao et. al.PubMed 32973960
- Molecular cancer therapeutics 19:1110-11222020Decitabine Response in Breast Cancer Requires Efficient Drug Processing and Is Not Limited by Multidrug Resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargaret L Dahn,Brianne M Cruickshank,Ainsleigh J Jackson,Cheryl Dean,Ryan W Holloway,Steven R Hall,Krysta M Coyle,Hillary Maillet,David M Waisman,Kerry B Goralski,Carman A Giacomantonio,Ian C G Weaver,Paola MarcatoPubMed 32156786
- Cancers 11:2019Role of Damage DNA-Binding Protein 1 in Pancreatic Cancer Progression and Chemoresistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiyin Zhang et. al.PubMed 31842285
- British journal of cancer 116:609-6192017Exosomes confer chemoresistance to pancreatic cancer cells by promoting ROS detoxification and miR-155-mediated suppression of key gemcitabine-metabolising enzyme, DCK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGirijesh Kumar Patel et. al.PubMed 28152544
- Nucleic acids research 44:9719-97322016The C/ebp-Atf response element (CARE) location reveals two distinct Atf4-dependent, elongation-mediated mechanisms for transcriptional induction of aminoacyl-tRNA synthetase genes in response to amino acid limitation.
- Leukemia 31:40-502016Systematic chemical and molecular profiling of MLL-rearranged infant acute lymphoblastic leukemia reveals efficacy of romidepsin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM N Cruickshank,J Ford,L C Cheung,J Heng,S Singh,J Wells,T W Failes,G M Arndt,N Smithers,R K Prinjha,D Anderson,K W Carter,A M Gout,T Lassmann,J O'Reilly,C H Cole,R S Kotecha,U R KeesPubMed 27443263
- Oncotarget 7:21812-242016Insights from HuR biology point to potential improvement for second-line ovarian cancer therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Hung Huang,Weidan Peng,Narumi Furuuchi,James B DuHadaway,Masaya Jimbo,Andrea Pirritano,Charles J Dunton,Gary S Daum,Benjamin E Leiby,Jonathan R Brody,Janet A SawickiPubMed 26943573
- Oncology reports 33:383-902014Establishment of human pancreatic cancer gemcitabine‑resistant cell line with ribonucleotide reductase overexpression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCongfei Wang,Weiwei Zhang,Mingjuan Fu,Aiqin Yang,Heguang Huang,Jieming XiePubMed 25394408
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DCK aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P27707
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Phosphorylates the deoxyribonucleosides deoxycytidine, deoxyguanosine and deoxyadenosine (PubMed:12808445, PubMed:18377927, PubMed:19159229, PubMed:1996353, PubMed:20614893, PubMed:20637175). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents (PubMed:12808445).
Alternative names
Deoxycytidine kinase, dCK, Deoxyadenosine kinase, Deoxyguanosine kinase, DCK
Recommended products
Rabbit Polyclonal DCK antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DCK aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DCK aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P27707
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-DCK antibody (ab151966)
12% SDS PAGE
All lanes: Western blot - Anti-DCK antibody (ab151966) at 1/10000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Lane 4: HepG2 whole cell lysate at 30 µg
Predicted band size: 31 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody (ab151966)
Immunohistochemical analysis of paraffin embedded Human Saos2 xenograft tissue labeling cytosolic DCK with ab151966 at a 1/500 dilution.
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