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AB118994

Anti-DCK antibody [OTI16E12]

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Mouse Monoclonal DCK antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DCK.

View Alternative Names

Deoxycytidine kinase, dCK, Deoxyadenosine kinase, Deoxyguanosine kinase, DCK

4 Images
Western blot - Anti-DCK antibody [OTI16E12] (AB118994)
  • WB

Unknown

Western blot - Anti-DCK antibody [OTI16E12] (AB118994)

All lanes:

Western blot - Anti-DCK antibody [OTI16E12] (ab118994) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cells transfected with pCMV6-ENTRY DCK cDNA at 5 µg

Predicted band size: 31 kDa

false

Flow Cytometry (Intracellular) - Anti-DCK antibody [OTI16E12] (AB118994)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-DCK antibody [OTI16E12] (AB118994)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either DCK overexpress plasmid (Red) or empty vector control plasmid (Blue) were stained for DCK using ab118994 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-DCK antibody [OTI16E12] (AB118994)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-DCK antibody [OTI16E12] (AB118994)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DCK using ab118994 (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry (Intracellular) - Anti-DCK antibody [OTI16E12] (AB118994)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-DCK antibody [OTI16E12] (AB118994)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab118994 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI16E12

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human DCK.

P27707

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Recombinant full length protein": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

The clone number has been updated from 16E12 to OTI16E12, both clone numbers name the same clone.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant by affinity chromatography
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylates the deoxyribonucleosides deoxycytidine, deoxyguanosine and deoxyadenosine (PubMed : 12808445, PubMed : 18377927, PubMed : 19159229, PubMed : 1996353, PubMed : 20614893, PubMed : 20637175). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents (PubMed : 12808445).
See full target information DCK
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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