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AB106639

Anti-DCLK2 antibody

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(4 Publications )

Rabbit Polyclonal DCLK2 antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DCLK2.
3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCLK2 antibody (AB106639)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCLK2 antibody (AB106639)

ab106639 at 5μg/ml, staining DCLK2 in Rat brain, using Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

Western blot - Anti-DCLK2 antibody (AB106639)
  • WB

Unknown

Western blot - Anti-DCLK2 antibody (AB106639)

Western Blot of rat brain tissue lysate labeling DCLK2 with Anti-DCLK2 antibody (ab106639) at (1) 1 and (2) 2μg/ml.

All lanes:

Western blot - Anti-DCLK2 antibody (ab106639)

Predicted band size: 26 kDa,83 kDa

false

Western blot - Anti-DCLK2 antibody (AB106639)
  • WB

Unknown

Western blot - Anti-DCLK2 antibody (AB106639)

Lane 1:

Western blot - Anti-DCLK2 antibody (ab106639) at 1 µg/mL

Lane 2:

Western blot - Anti-DCLK2 antibody (ab106639) at 2 µg/mL

All lanes:

Rat brain tissue lysate at 15 µg

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human DCLK2. The exact immunogen used to generate this antibody is proprietary information.

Q8N568

Specificity

Based on analysis of the immunogen sequence, we would expect this antibody to react with all three described isoforms of human DCLK2 (Q8N568-1, -2,-3) isoforms 1 and 3 of rat DCLK2 (Q5MPA9-1,-3) but not isoform 2 (Q5MPA9-2), and isoforms 1, 2, and 3 (Q6PGN3-1,-2,-3) of mouse DCLK2 but not isoform 4, 5, and 6 (Q6PGN3-4,-5,-6).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab106639 is purified by affinity chromatography via a peptide column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).
See full target information DCLK2

Alternative Names

DCAMKL2, DCDC3B, DCK2, DCLK2, Serine/threonine-protein kinase DCLK2, CaMK-like CREB regulatory kinase 2, Doublecortin domain-containing protein 3B, Doublecortin-like and CAM kinase-like 2, Doublecortin-like kinase 2, CL2, CLICK-II, CLICK2
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Publications (4)

Recent publications for all applications. Explore the full listand refine your search

Nature communications 13:6040 PubMed36229455

2022

Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system.

Applications

Unspecified application

Species

Unspecified reactive species

Noemie Vilallongue,Julia Schaeffer,Anne-Marie Hesse,Céline Delpech,Béatrice Blot,Antoine Paccard,Elise Plissonnier,Blandine Excoffier,Yohann Couté,Stephane Belin,Homaira Nawabi

Biology 11: PubMed35205114

2022

Doublecortin in the Fish Visual System, a Specific Protein of Maturing Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Laura DeOliveira-Mello,Isabel Vicente,Veronica Gonzalez-Nunez,Adrian Santos-Ledo,Almudena Velasco,Rosario Arévalo,Juan M Lara,Andreas F Mack

The Journal of biological chemistry 296:100082 PubMed33199366

2020

The X-linked intellectual disability gene product and E3 ubiquitin ligase KLHL15 degrades doublecortin proteins to constrain neuronal dendritogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianing Song,Ronald A Merrill,Andrew Y Usachev,Stefan Strack

The EMBO journal 35:302-18 PubMed26758546

2016

Microtubule-binding protein doublecortin-like kinase 1 (DCLK1) guides kinesin-3-mediated cargo transport to dendrites.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Lipka,Lukas C Kapitein,Jacek Jaworski,Casper C Hoogenraad
View all publications
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Product promise

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