Anti-Dcp1a antibody [EPR13822]

• WB

Supplier Data

Western blot - Anti-Dcp1a antibody [EPR13822] (AB183709)

All lanes:

Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 42 kDa,63 kDa,72 kDa,98 kDa

Observed band size: 75 kDa

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• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (AB183709)

ab183709 staining Dcp1a in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Control : PBS only

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp1a antibody [EPR13822] (AB183709)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Dcp1a with ab183709 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Dcp1a antibody [EPR13822] (AB183709)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Dcp1a with ab183709 at 1/150 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (AB183709)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dcp1a with ab183709 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).

• WB

Supplier Data

Western blot - Anti-Dcp1a antibody [EPR13822] (AB183709)

All lanes:

Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/10000 dilution

All lanes:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 63 kDa

Observed band size: 75 kDa

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• WB

Lab

Western blot - Anti-Dcp1a antibody [EPR13822] (AB183709)

Lanes 1 - 3 : Merged signal (red and green). Green - ab183709 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab183709 was shown to specifically react with DCP1A in wild-type HEK-293 cells as signal was lost in DCP1A knockout cell line ab261857 (knockout cell lysate ab261665). Wild-type and DCP1A knockout samples were subjected to SDS-PAGE. ab183709 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

DCP1A knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human DCP1A knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-dcp1a-knockout-hek-293-cell-line-ab261857'>ab261857</a>)

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 63 kDa

Observed band size: 75 kDa

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