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Rabbit Recombinant Monoclonal Dcp2/TDT antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Dcp2/TDT antibody [EPR25378-93] (AB288158), expandable thumbnail
  • Immunoprecipitation - Anti-Dcp2/TDT antibody [EPR25378-93] (AB288158), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Dcp2/TDT antibody [EPR25378-93] (AB288158), expandable thumbnail
  • Western blot - Anti-Dcp2/TDT antibody [EPR25378-93] (AB288158), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Dcp2/TDT antibody [EPR25378-93] (AB288158), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Not recommended
Tested
Mouse
Not recommended
Expected
Tested
Not recommended
Tested
Rat
Not recommended
Expected
Tested
Not recommended
Expected

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed:12218187, PubMed:12417715, PubMed:12923261, PubMed:21070968, PubMed:28002401, PubMed:31875550). Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed:12486012, PubMed:12923261, PubMed:21070968, PubMed:28002401, PubMed:31875550). Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (PubMed:14527413). Plays a role in replication-dependent histone mRNA degradation (PubMed:18172165). Has higher activity towards mRNAs that lack a poly(A) tail (PubMed:21070968). Has no activity towards a cap structure lacking an RNA moiety (PubMed:21070968). The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping (PubMed:28002401). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573).

Alternative names

NUDT20, DCP2, m7GpppN-mRNA hydrolase, Nucleoside diphosphate-linked moiety X motif 20, mRNA-decapping enzyme 2, Nudix motif 20, hDpc

Recommended products

Rabbit Recombinant Monoclonal Dcp2/TDT antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25378-93
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158), expandable thumbnail

    Western blot - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Fresh lysates were used in this WB.

    The observed MW/expression profile is consistent with what has been described in the literature (PMID: 21070968 & Unipro). The ~49 kDa band is the long isoform, while ~44 kDa band is the short isoform.

    Exposure time: 37 seconds

    All lanes: Western blot - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa

    Observed band size: 44 kDa, 49 kDa

  • Immunoprecipitation - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158), expandable thumbnail

    Immunoprecipitation - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158)

    Dcp2/TDT was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug with ab288158 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288158 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab288158 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab288158 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158)

    Predicted band size: 48 kDa

    Observed band size: 49 kDa

  • Flow Cytometry (Intracellular) - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Dcp2/TDT with ab288158 at 1/50 dilution (1ug)(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158), expandable thumbnail

    Western blot - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW/expression profile is consistent with what has been described in the literature (PMID: 21070968).

    Low expression: skeletal muscle.

    Exposure time: Lane 1-5: 37 seconds Lane 6-8: 70 seconds

    All lanes: Western blot - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Lane 3: Mouse testis tissue lysate at 20 µg

    Lane 4: Mouse brain tissue lysate at 20 µg

    Lane 5: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 6: Rat testis tissue lysate at 20 µg

    Lane 7: Rat brain tissue lysate at 20 µg

    Lane 8: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa

    Observed band size: 44 kDa, 49 kDa

  • Flow Cytometry (Intracellular) - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Dcp2/TDT antibody [EPR25378-93] (ab288158)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Dcp2/TDT with ab288158 at 1/50 dilution (1ug)(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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