Anti-DCTN1/p150-glued antibody [12/P150GLUED] - BSA and Azide Free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [12/P150GLUED] - BSA and Azide Free (AB302965)

This data was developed using ab302964, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DCTN1/p150-glued with ab302964 at 1/10000 (0.119 μg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse cerebrum. The section was incubated with ab302964 for 30 mins at room temperature, followed by anti-Mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody Leica DS9800 (Bond™ Polymer Refine Detection kit). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [12/P150GLUED] - BSA and Azide Free (AB302965)

This data was developed using ab302964, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling DCTN1/p150-glued with ab302964 at 1/10000 (0.119 μg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse cerebellum. The section was incubated with ab302964 for 30 mins at room temperature, followed by anti-Mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody Leica DS9800 (Bond™ Polymer Refine Detection kit). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• WB

Supplier Data

Western blot - Anti-DCTN1/p150-glued antibody [12/P150GLUED] - BSA and Azide Free (AB302965)

This data was developed using ab302964, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile observed is consistent with what has been described in the literature (PMID : 8856662). Exposure time : Lane 1, 4 : 3 minutes Lane 2 : 45 seconds Lane 3, 5, 6 : 114 seconds

All lanes:

Western blot - Anti-DCTN1/p150-glued antibody [12/P150GLUED] (<a href='/en-us/products/primary-antibodies/dctn1-p150-glued-antibody-12-p150glued-ab302964'>ab302964</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse cerebellum tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat cerebellum tissue lysate at 20 µg

Lane 6:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

Observed band size: 150 kDa

false

• WB

Supplier Data

Western blot - Anti-DCTN1/p150-glued antibody [12/P150GLUED] - BSA and Azide Free (AB302965)

This data was developed using ab302964, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 48 seconds

All lanes:

Western blot - Anti-DCTN1/p150-glued antibody [12/P150GLUED] (<a href='/en-us/products/primary-antibodies/dctn1-p150-glued-antibody-12-p150glued-ab302964'>ab302964</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

Hela transfected with siRNA specifically targeti DCTN1, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/2000 dilution

Observed band size: 150 kDa

false

Exposure time: 48s