AB96004

Anti-DCTN1/p150-glued antibody

Name: Anti-DCTN1/p150-glued antibody (ab96004) | Abcam
Brand: Abcam Limited
SKU: AB96004

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(3 Publications)

Rabbit Polyclonal DCTN1/p150-glued antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DCTN1 aa 1200-1250.

View Alternative Names

Dynactin subunit 1, 150 kDa dynein-associated polypeptide, DAP-150, p135, p150-glued, DP-150, DCTN1

9 Images

Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody (AB96004)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody (AB96004)

Immunofluorescence analysis of methanol-fixed HeLa cells, using ab96004 at 1/200 dilution. Lower image costained with Hoerchst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

Immunofluorescence analysis of methanol-fixed HeLa, using ab96004 antibody (Green) at 1 : 500 dilution. Alpha-tubulin filaments were labeled (Red) at 1 : 2000.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

Immunohistochemical analysis of paraffin-embedded Rat brain, using ab96004 at 1/500 dilution. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

All lanes:

Western blot - Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution

Lane 1:

U87-MG cell lysates at 30 µg

Lane 2:

SK-N-SH cell lysates at 30 µg

Lane 3:

IMR32 cell lysates at 30 µg

Lane 4:

SK-N-AS cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 141 kDa

false

Unknown

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

5% SDS PAGE

All lanes:

Western blot - Anti-DCTN1/p150-glued antibody (ab96004) at 1/3000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 141 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

Immunohistochemical analysis of paraffin-embedded ovarian cancer, using ab96004 at 1/100 dilution. Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Supplier Data

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

All lanes:

Western blot - Anti-DCTN1/p150-glued antibody (ab96004) at 1/1000 dilution

Lane 1:

Mouse brain at 50 µg

Lane 2:

Mouse lung at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 141 kDa

false

Supplier Data

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

All lanes:

Western blot - Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution

Lane 1:

Jurkat cell lysate at 30 µg

Lane 2:

Raji cell lysate at 30 µg

Lane 3:

NCI-H929 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 141 kDa

false

Supplier Data

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

All lanes:

Western blot - Anti-DCTN1/p150-glued antibody (ab96004) at 1/2000 dilution

Lane 1:

Mouse brain at 50 µg

Lane 2:

Rat brain at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 141 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human DCTN1 aa 1200-1250. The exact immunogen used to generate this antibody is proprietary information.

Q14203

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed : 25185702). Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon (PubMed : 23874158). Plays a role in metaphase spindle orientation (PubMed : 22327364). Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole (PubMed : 23386061). Plays a role in primary cilia formation (PubMed : 25774020).

See full target information DCTN1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:328 PubMed40263294

2025

Opposing roles for GSK3β and ERK1-dependent phosphorylation of huntingtin during neuronal dysfunction and cell death in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Krzystek,Rasika Rathnayake,Jia Zeng,Jing Huang,Gary Iacobucci,Michael C Yu,Shermali Gunawardena

Autophagy 19:1199-1220 PubMed36048753

2022

HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Krzystek,Joseph A White,Rasika Rathnayake,Layne Thurston,Hayley Hoffmar-Glennon,Yichen Li,Shermali Gunawardena

Evidence-based complementary and alternative medicine : eCAM 2022:6400517 PubMed35310029

2022

Modified Yuejuwan Inhibited Cholesterol Accumulation and Inflammation in THP-1 Macrophage-Derived Foam Cells by Inhibiting the Activity of the TRIM37/TRAF2/NF-B Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingtai Gui,Lei Yao,Bo Lu,Jianhua Li,Jing Wang,Xunjie Zhou,Deyu Fu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DCTN1/p150-glued antibody

Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody (AB96004)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise