Rabbit Polyclonal DCTN1/p150-glued antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DCTN1 aa 1200-1250.
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- Autophagy 19:1199-12202022HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas J Krzystek,Joseph A White,Rasika Rathnayake,Layne Thurston,Hayley Hoffmar-Glennon,Yichen Li,Shermali GunawardenaPubMed 36048753
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DCTN1 aa 1200-1250. The exact immunogen used to generate this antibody is proprietary information. Database link Q14203
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/500 | Notes - |
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed:25185702). Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon (PubMed:23874158). Plays a role in metaphase spindle orientation (PubMed:22327364). Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole (PubMed:23386061). Plays a role in primary cilia formation (PubMed:25774020).
Alternative names
Dynactin subunit 1, 150 kDa dynein-associated polypeptide, DAP-150, p135, p150-glued, DP-150, DCTN1
Recommended products
Rabbit Polyclonal DCTN1/p150-glued antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DCTN1 aa 1200-1250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DCTN1 aa 1200-1250. The exact immunogen used to generate this antibody is proprietary information. Database link Q14203
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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9 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (ab96004)
Immunohistochemical analysis of paraffin-embedded Rat brain, using ab96004 at 1/500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
All lanes: Western blot - Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution
Lane 1: Jurkat cell lysate at 30 µg
Lane 2: Raji cell lysate at 30 µg
Lane 3: NCI-H929 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 141 kDa
Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
All lanes: Western blot - Anti-DCTN1/p150-glued antibody (ab96004) at 1/1000 dilution
Lane 1: Mouse brain at 50 µg
Lane 2: Mouse lung at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 141 kDa
Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
All lanes: Western blot - Anti-DCTN1/p150-glued antibody (ab96004) at 1/2000 dilution
Lane 1: Mouse brain at 50 µg
Lane 2: Rat brain at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 141 kDa
Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
All lanes: Western blot - Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution
Lane 1: U87-MG cell lysates at 30 µg
Lane 2: SK-N-SH cell lysates at 30 µg
Lane 3: IMR32 cell lysates at 30 µg
Lane 4: SK-N-AS cell lysates at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 141 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (ab96004)
Immunofluorescence analysis of methanol-fixed HeLa, using ab96004 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000.
Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
5% SDS PAGE
All lanes: Western blot - Anti-DCTN1/p150-glued antibody (ab96004) at 1/3000 dilution
All lanes: Jurkat whole cell lysate at 30 µg
Predicted band size: 141 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody (ab96004)
Immunofluorescence analysis of methanol-fixed HeLa cells, using ab96004 at 1/200 dilution. Lower image costained with Hoerchst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (ab96004)
Immunohistochemical analysis of paraffin-embedded ovarian cancer, using ab96004 at 1/100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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