Rabbit Recombinant Monoclonal DCTN1/p150-glued antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR23547-2] (AB246505), expandable thumbnail](https://content.abcam.com/products/images/dctn1-p150-glued-antibody-epr23547-2-ab246505--immunoprecipitation-img11025.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR23547-2] (AB246505)")
![Western blot - Anti-DCTN1/p150-glued antibody [EPR23547-2] (AB246505), expandable thumbnail](https://content.abcam.com/products/images/dctn1-p150-glued-antibody-epr23547-2-ab246505--western-blot-img13024.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DCTN1/p150-glued antibody [EPR23547-2] (AB246505)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Mouse | Expected | Tested | Not recommended |
| Rat | Expected | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed:25185702). Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon (PubMed:23874158). Plays a role in metaphase spindle orientation (PubMed:22327364). Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole (PubMed:23386061). Plays a role in primary cilia formation (PubMed:25774020).
Alternative names
Dynactin subunit 1, 150 kDa dynein-associated polypeptide, DAP-150, p135, p150-glued, DP-150, DCTN1
Recommended products
Rabbit Recombinant Monoclonal DCTN1/p150-glued antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.
Alternative names
Dynactin subunit 1, 150 kDa dynein-associated polypeptide, DAP-150, p135, p150-glued, DP-150, DCTN1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR23547-2
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505), expandable thumbnail](https://content.abcam.com/products/images/dctn1-p150-glued-antibody-epr23547-2-ab246505--immunoprecipitation-img11025.jpg "Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)")
Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)
DCTN1/p150-glued was immunoprecipitated from 0.35 mg human brain tissue lysate with ab246505 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246505 at 1/1000 dilution (0.573 μg/ml). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human brain tissue lysate 10μg
Lane 2: ab246505 IP in human brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab246505 in human brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
The expression profile observed is consistent with what has been described in the literature (PMID: 8856662).
All lanes: Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)
Predicted band size: 141 kDa
Observed band size: 150 kDa
![Western blot - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505), expandable thumbnail](https://content.abcam.com/products/images/dctn1-p150-glued-antibody-epr23547-2-ab246505--western-blot-img13024.jpg "Western blot - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)")
Western blot - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 8856662, 27365401).
Negative control: Human intestine (Roodink, I. (2009). Tackling the vascular heterogeneity issue in tumors: identification of novel targets for tumor therapy (Doctoral dissertation)).
Lane 3 blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-DCTN1/p150-glued antibody [EPR23547-2] (ab246505) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 40 µg
Lane 2: Human large intestine tissue lysate at 40 µg
Lane 3: Human cerebellum tissue lysate at 40 µg
Lane 4: Mouse brain tissue lysate at 40 µg
Lane 5: Mouse cerebellum tissue lysate at 40 µg
Lane 6: Rat brain tissue lysate at 40 µg
SecondaryAll lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 150 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com