Anti-DCTN1/p150-glued antibody [EPR26464-28]

14 product images

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  Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling DCTN1/p150-glued with ab302629 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

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  Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling DCTN1/p150-glued with ab302629 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

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  Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  
  Exposure time: 114 seconds

  All lanes: Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629) at 1/1000 dilution

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), transfected with scrambled siRNA control, whole cell lysate 20 µg

  Lane 2: Hela transfected with siRNA specifically targeti DCTN1, whole cell lysate 20

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 150 kDa

  Exposure time: 114s

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Immunohistochemical analysis of paraffin-embedded Mouse spinal cord tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse spinal cord (PMID: 23408943, 17122035 ). The section was incubated with ab302629 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on mouse liver (PMID: 1836789). The section was incubated with ab302629 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling DCTN1/p150-glued with ab302629 at 1/50 (10.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing mainly cytoplasmic staining in HeLa cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling DCTN1/p150-glued with ab302629 at 1/50 (10.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

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  Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spinal cord (fresh) tissue labeling DCTN1/p150-glued with ab302629 at 1/100 (1.078 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse spinal cord is observed. The nuclear counterstain was DAPI (Blue).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p>

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  Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling DCTN1/p150-glued with ab302629 at 1/100 (1.078 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on mouse liver (PMID: 1836789) is observed. The nuclear counterstain was DAPI (Blue).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p>

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Immunohistochemical analysis of paraffin-embedded Mouse dorsal root ga tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse dorsal root ganglion (PMID: 23874158). The section was incubated with ab302629 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum (PMID: 17122035). The section was incubated with ab302629 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  All lanes: Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629) at 1/1000 dilution

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 µg

  Lane 2: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 20 µg

  Lane 3: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 20 µg

  Lane 4: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 20 µg

  Lane 5: Mouse brain tissue lysate 20 µg

  Lane 6: Mouse cerebellum tissue lysate 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 150 kDa

  Exposure time: 3min

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  Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  DCTN1/p150-glued was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 µg with ab302629 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302629 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 µg
  
  Lane 2: ab302629 IP in Neuro-2a whole cell lysate
  
  Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab302629 in Neuro-2a whole cell lysate

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  All lanes: Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629) at 1/30 dilution

  All lanes: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 3min

• 

  Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)

  DCTN1/p150-glued was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg with ab302629 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302629 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution..

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg
  
  Lane 2: ab302629 IP in HeLa whole cell lysate
  
  Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab302629 in HeLa whole cell lysate

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  All lanes: Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629) at 1/30 dilution

  All lanes: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366)

  Predicted band size: 141 kDa

  Exposure time: 3min