Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)

Rabbit Recombinant Monoclonal DCTN1/p150-glued antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB and reacts with Mouse, Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (AB302630), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-Fr	IHC-P	WB
Human	Tested	Tested	Tested	Expected	Not recommended	Tested
Mouse	Tested	Tested	Tested	Tested	Tested	Tested
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Target data

See full target information DCTN1

Function

Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed:25185702). Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon (PubMed:23874158). Plays a role in metaphase spindle orientation (PubMed:22327364). Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole (PubMed:23386061). Plays a role in primary cilia formation (PubMed:25774020).

Alternative names

Dynactin subunit 1, 150 kDa dynein-associated polypeptide, DAP-150, p135, p150-glued, DP-150, DCTN1

Recommended products

Rabbit Recombinant Monoclonal DCTN1/p150-glued antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB and reacts with Mouse, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR26464-28
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling DCTN1/p150-glued with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling DCTN1/p150-glued with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer
Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 114 seconds
All lanes: Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), transfected with scrambled siRNA control, whole cell lysate 20 µg
Lane 2: Hela transfected with siRNA specifically targeti DCTN1, whole cell lysate 20
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 150 kDa
Exposure time: 114s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spinal cord tissue labeling DCTN1/p150-glued with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/1000 (0.539 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse spinal cord (PMID: 23408943, 17122035 ). The section was incubated with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling DCTN1/p150-glued with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/1000 (0.539 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: no staining on mouse liver (PMID: 1836789). The section was incubated with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling DCTN1/p150-glued with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/50 (10.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing mainly cytoplasmic staining in HeLa cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling DCTN1/p150-glued with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/50 (10.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spinal cord (fresh) tissue labeling DCTN1/p150-glued with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/100 (1.078 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse spinal cord is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling DCTN1/p150-glued with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/100 (1.078 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on mouse liver (PMID: 1836789) is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse dorsal root ga tissue labeling DCTN1/p150-glued with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/1000 (0.539 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse dorsal root ganglion (PMID: 23874158). The section was incubated with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling DCTN1/p150-glued with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/1000 (0.539 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse cerebrum (PMID: 17122035). The section was incubated with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 µg
Lane 2: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 20 µg
Lane 3: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 20 µg
Lane 5: Mouse brain tissue lysate 20 µg
Lane 6: Mouse cerebellum tissue lysate 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 150 kDa
Exposure time: 3min
Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
DCTN1/p150-glued was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 µg with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 µg

Lane 2: Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 IP in Neuro-2a whole cell lysate

Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629) at 1/30 dilution
All lanes: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3min
Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (BSA and Azide free) (ab302630)
This data was developed using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629, the same antibody clone in a different buffer formulation.
DCTN1/p150-glued was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg with Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution..
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg

Lane 2: Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 IP in HeLa whole cell lysate

Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (Anti-DCTN1/p150-glued antibody [EPR26464-28] ab302629) at 1/30 dilution
All lanes: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366)
Predicted band size: 141 kDa
Exposure time: 3min