Rabbit Recombinant Monoclonal DCTN3 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (AB232440), expandable thumbnail](https://content.abcam.com/products/images/dctn3-antibody-epr5097-bsa-and-azide-free-ab232440--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87781.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (AB232440)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (AB232440), expandable thumbnail](https://content.abcam.com/products/images/dctn3-antibody-epr5097-bsa-and-azide-free-ab232440--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45412.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (AB232440)")
![Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (AB232440), expandable thumbnail](https://content.abcam.com/products/images/dctn3-antibody-epr5097-bsa-and-azide-free-ab232440--immunocytochemistry-immunofluorescence-img15414.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (AB232440)")
![OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (AB232440), expandable thumbnail](https://content.abcam.com/products/images/dctn3-antibody-epr5097-bsa-and-azide-free-ab232440--oi-rd-scanning-img139228.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (AB232440)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Predicted | Expected | Predicted |
| Rat | Predicted | Expected | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
1 products for Alternative Product
Target data
Function
Together with dynein may be involved in spindle assembly and cytokinesis.
Alternative names
Dynactin subunit 3, Dynactin complex subunit 22 kDa subunit, p22, DCTN3, DCTN22
Recommended products
Rabbit Recombinant Monoclonal DCTN3 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples.
Alternative names
Dynactin subunit 3, Dynactin complex subunit 22 kDa subunit, p22, DCTN3, DCTN22
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR5097
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab232440 is the carrier-free version of Anti-DCTN3 antibody [EPR5097] ab124674.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440), expandable thumbnail](https://content.abcam.com/products/images/dctn3-antibody-epr5097-bsa-and-azide-free-ab232440--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87781.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
Immunohistochemical staining of DCTN3 in paraffin embedded human breast tissue by unpurified Anti-DCTN3 antibody [EPR5097] ab124674 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DCTN3 antibody [EPR5097] ab124674).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440), expandable thumbnail](https://content.abcam.com/products/images/dctn3-antibody-epr5097-bsa-and-azide-free-ab232440--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45412.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
Immunohistochemical staining of paraffin embedded human pancreas with purified Anti-DCTN3 antibody [EPR5097] ab124674 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DCTN3 antibody [EPR5097] ab124674).
![Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440), expandable thumbnail](https://content.abcam.com/products/images/dctn3-antibody-epr5097-bsa-and-azide-free-ab232440--immunocytochemistry-immunofluorescence-img15414.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)")
Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
Immunofluorescence staining of BxPC-3 cells with purified Anti-DCTN3 antibody [EPR5097] ab124674 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified Anti-DCTN3 antibody [EPR5097] ab124674 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab15120) at a dilution of 1/500.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DCTN3 antibody [EPR5097] ab124674).
![OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440), expandable thumbnail](https://content.abcam.com/products/images/dctn3-antibody-epr5097-bsa-and-azide-free-ab232440--oi-rd-scanning-img139228.jpg "OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)")
OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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