Rabbit Recombinant Monoclonal DCTN4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-DCTN4 antibody [EPR11223] (AB170107), expandable thumbnail](https://content.abcam.com/products/images/dctn4-antibody-epr11223-ab170107--western-blot-img107584.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DCTN4 antibody [EPR11223] (AB170107)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN4 antibody [EPR11223] (AB170107), expandable thumbnail](https://content.abcam.com/products/images/dctn4-antibody-epr11223-ab170107--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76690.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN4 antibody [EPR11223] (AB170107)")
![Immunocytochemistry/ Immunofluorescence - Anti-DCTN4 antibody [EPR11223] (AB170107), expandable thumbnail](https://content.abcam.com/products/images/dctn4-antibody-epr11223-ab170107--immunocytochemistry-immunofluorescence-img116968.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DCTN4 antibody [EPR11223] (AB170107)")
Publications
Filter by
- NPJ Parkinson's disease 9:352023Deficiency of Perry syndrome-associated p150 in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Yu,Xuan Yang,Jiayin Zheng,Carmelo Sgobio,Lixin Sun,Huaibin CaiPubMed 36879021
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Use of an HRP/AP polymerized secondary antibody recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules.
Alternative names
Dynactin subunit 4, Dyn4, Dynactin subunit p62, DCTN4
Recommended products
Rabbit Recombinant Monoclonal DCTN4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR11223
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-DCTN4 antibody [EPR11223] (ab170107), expandable thumbnail](https://content.abcam.com/products/images/dctn4-antibody-epr11223-ab170107--western-blot-img107584.jpg "Western blot - Anti-DCTN4 antibody [EPR11223] (ab170107)")
Western blot - Anti-DCTN4 antibody [EPR11223] (ab170107)
All lanes: Western blot - Anti-DCTN4 antibody [EPR11223] (ab170107) at 1/1000 dilution
Lane 1: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg
Lane 2: K562 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN4 antibody [EPR11223] (ab170107), expandable thumbnail](https://content.abcam.com/products/images/dctn4-antibody-epr11223-ab170107--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76690.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN4 antibody [EPR11223] (ab170107)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN4 antibody [EPR11223] (ab170107)
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling DCTN4 with ab170107 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-DCTN4 antibody [EPR11223] (ab170107), expandable thumbnail](https://content.abcam.com/products/images/dctn4-antibody-epr11223-ab170107--immunocytochemistry-immunofluorescence-img116968.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DCTN4 antibody [EPR11223] (ab170107)")
Immunocytochemistry/ Immunofluorescence - Anti-DCTN4 antibody [EPR11223] (ab170107)
Immunofluorescence analysis of HeLa cells labeling DCTN4 with ab170107 at a 1/100 dilution (red) plus Dapi staining (blue).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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