AB181233

Anti-DCUN1D1 antibody [EPR13492]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal DCUN1D1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

DCN1, DCUN1L1, RP42, SCCRO, DCUN1D1, DCN1-like protein 1, DCNL1, DCUN1 domain-containing protein 1, Defective in cullin neddylation protein 1-like protein 1, Squamous cell carcinoma-related oncogene

2 Images

Supplier Data

Western blot - Anti-DCUN1D1 antibody [EPR13492] (AB181233)

All lanes:

Western blot - Anti-DCUN1D1 antibody [EPR13492] (ab181233) at 1/20000 dilution

Lane 1:

A549 cell lysate at 20 µg

Lane 2:

HT29 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

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Supplier Data

Immunoprecipitation - Anti-DCUN1D1 antibody [EPR13492] (AB181233)

Immunoprecipitation analysis of HeLa cells labeling DCUN1D1 using ab181233 at 1/50 dilution. A Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-DCUN1D1 antibody [EPR13492] (ab181233)

Predicted band size: 30 kDa

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Carrier free

Anti-DCUN1D1 antibody [EPR13492] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13492

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of an E3 ubiquitin ligase complex for neddylation (PubMed : 18826954). Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes (PubMed : 19617556, PubMed : 23201271, PubMed : 23401859, PubMed : 26906416). Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity (PubMed : 25349211, PubMed : 28581483). Acts also as an oncogene facilitating malignant transformation and carcinogenic progression (By similarity).

See full target information DCUN1D1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:28 PubMed34824636

2021

MicroRNA-3148 inhibits glioma by decreasing and inhibiting the NF-kB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qianghua Xu,Xiao Chen,Bin Chen

Experimental and therapeutic medicine 16:1000-1008 PubMed30116351

2018

MicroRNA-302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjie Jiang,Ruijie Hou,Shaoping Li,Shaoru Li,Ge Dang

International journal of molecular medicine 42:779-788 PubMed29750306

2018

MicroRNA‑195 inhibits cell proliferation, migration and invasion by targeting defective in cullin neddylation 1 domain containing 1 in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyan Zhong,Hui Yuan,Xiangqian Xu,Shoufang Kong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DCUN1D1 antibody [EPR13492]

Western blot - Anti-DCUN1D1 antibody [EPR13492] (AB181233)

Immunoprecipitation - Anti-DCUN1D1 antibody [EPR13492] (AB181233)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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Product promise