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AB276073

Anti-DCUN1D2 antibody [001] - BSA and Azide free

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Rabbit Recombinant Monoclonal DCUN1D2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DCUN1D2 aa 1 to C-terminus.

View Alternative Names

C13orf17, DCUN1L2, DCUN1D2, DCN1-like protein 2, DCNL2, DCUN1 domain-containing protein 2, Defective in cullin neddylation protein 1-like protein 2

2 Images
Immunoprecipitation - Anti-DCUN1D2 antibody [001] - BSA and Azide free (AB276073)
  • IP

Unknown

Immunoprecipitation - Anti-DCUN1D2 antibody [001] - BSA and Azide free (AB276073)

DCUN1D2 was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) (Lane A), A549 (Human lung carcinoma cell line) (Lane B) and HuT 78 (human cutaneous T lymphocyte Sezary Syndrome cell line) whole cell lysates with 2 μL ab276073 and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitates using ab276073 at 1/100 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.

Developed using the odssey technique.
Performed under reducing conditions.

All lanes:

Immunoprecipitation - Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073)

Predicted band size: 30 kDa

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Western blot - Anti-DCUN1D2 antibody [001] - BSA and Azide free (AB276073)
  • WB

Unknown

Western blot - Anti-DCUN1D2 antibody [001] - BSA and Azide free (AB276073)

Developed using the Odyssey technique.

Performed under reducing conditions.

All lanes:

Western blot - Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073) at 1/500 dilution

All lanes:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

001

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human DCUN1D2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6PH85

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>1-4 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Contributes to the neddylation of all cullins by transferring NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes and plays an essential role in the regulation of SCF (SKP1-CUL1-F-box protein)-type complexes activity.
See full target information DCUN1D2
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