Rabbit Recombinant Monoclonal DCUN1D2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DCUN1D2 aa 1 to C-terminus.
Images
![Immunoprecipitation - Anti-DCUN1D2 antibody [001] - BSA and Azide free (AB276073), expandable thumbnail](https://content.abcam.com/products/images/dcun1d2-antibody-001-bsa-and-azide-free-ab276073--immunoprecipitation-img10865.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-DCUN1D2 antibody [001] - BSA and Azide free (AB276073)")
![Western blot - Anti-DCUN1D2 antibody [001] - BSA and Azide free (AB276073), expandable thumbnail](https://content.abcam.com/products/images/dcun1d2-antibody-001-bsa-and-azide-free-ab276073--western-blot-img67096.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DCUN1D2 antibody [001] - BSA and Azide free (AB276073)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human DCUN1D2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PH85
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 1-4 μL/mg of lysate |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Target data
Function
Contributes to the neddylation of all cullins by transferring NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes and plays an essential role in the regulation of SCF (SKP1-CUL1-F-box protein)-type complexes activity.
Alternative names
C13orf17, DCUN1L2, DCUN1D2, DCN1-like protein 2, DCNL2, DCUN1 domain-containing protein 2, Defective in cullin neddylation protein 1-like protein 2
Recommended products
Rabbit Recombinant Monoclonal DCUN1D2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DCUN1D2 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human DCUN1D2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PH85
- Carrier free
- Yes
- Clone number
- 001
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073), expandable thumbnail](https://content.abcam.com/products/images/dcun1d2-antibody-001-bsa-and-azide-free-ab276073--immunoprecipitation-img10865.jpg "Immunoprecipitation - Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073)")
Immunoprecipitation - Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073)
DCUN1D2 was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) (Lane A), A549 (Human lung carcinoma cell line) (Lane B) and HuT 78 (human cutaneous T lymphocyte Sezary Syndrome cell line) whole cell lysates with 2 μL ab276073 and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitates using ab276073 at 1/100 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.
Developed using the odssey technique.
Performed under reducing conditions.All lanes: Immunoprecipitation - Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073)
Predicted band size: 30 kDa
![Western blot - Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073), expandable thumbnail](https://content.abcam.com/products/images/dcun1d2-antibody-001-bsa-and-azide-free-ab276073--western-blot-img67096.jpg "Western blot - Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073)")
Western blot - Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073)
Developed using the Odyssey technique.
Performed under reducing conditions.All lanes: Western blot - Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073) at 1/500 dilution
All lanes: HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 30 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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