Anti-DCXR antibody [4G4AF5]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-DCXR antibody [4G4AF5] (AB110283)

Immunocytochemistry image of ab110283 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110283 at 4 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the cell membrane.

• Flow Cyt

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Flow Cytometry - Anti-DCXR antibody [4G4AF5] (AB110283)

HL-60 cells were stained with 1 µg/mL ab110283 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

• WB

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Western blot - Anti-DCXR antibody [4G4AF5] (AB110283)

All lanes:

Western blot - Anti-DCXR antibody [4G4AF5] (ab110283) at 1 µg/mL

All lanes:

Human liver mitochondria at 20 µg

Secondary

All lanes:

Goat anti-mouse conjugated to AP

Predicted band size: 26 kDa

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