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AB117440

Anti-DDA1 antibody

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(1 Publication)

Rabbit Polyclonal DDA1 antibody. Suitable for ICC, WB, ICC/IF and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DDA1.

View Alternative Names

C19orf58, PCIA1, DDA1, DET1- and DDB1-associated protein 1, Placenta cross-immune reaction antigen 1, PCIA-1

3 Images
Immunocytochemistry - Anti-DDA1 antibody (AB117440)
  • ICC

Unknown

Immunocytochemistry - Anti-DDA1 antibody (AB117440)

Immunohistochemical analysis of mouse heart tissue, staining DDA1 with ab117440 at 5 µg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-DDA1 antibody (AB117440)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DDA1 antibody (AB117440)

Immunofluorescence of DDA1 in mouse heart tissue with ab117440 at 20 ug/mL.

Western blot - Anti-DDA1 antibody (AB117440)
  • WB

Unknown

Western blot - Anti-DDA1 antibody (AB117440)

All lanes:

Western blot - Anti-DDA1 antibody (ab117440) at 1 µg/mL

Lane 1:

Mouse heart tissue lysate at 15 µg

Lane 2:

Mouse heart tissue lysate at 15 µg with blocking peptide

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, ICC, WB

applications

Immunogen

Synthetic Peptide within Human DDA1. The exact immunogen used to generate this antibody is proprietary information.

Q9BW61

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DDA1 (DET1 and DDB1 associated 1) protein also known as DET1 and DDB1-associated protein 1 plays an important mechanical role in cellular processes. This protein has a molecular mass of approximately 12 kDa and is ubiquitously expressed in various tissues. DDA1 is involved in the ubiquitin-proteasome pathway acting primarily as an adaptor protein that facilitates the recruitment of substrates for ubiquitination. Its mechanical function contributes to the regulation of protein stability and degradation.
Biological function summary

DDA1 interacts with several other proteins to form part of the Cullin-RING E3 ubiquitin ligase (CRL) complex. This complex is important for tagging target proteins with ubiquitin marking them for degradation by the proteasome. DDA1's interaction with DET1 and DDB1 highlights its role in influencing cellular homeostasis and response to DNA damage. By participating in these interactions it helps maintain normal cell cycle progression and cellular response mechanisms.

Pathways

DDA1 contributes to both ubiquitin-mediated proteolysis and cell cycle regulation. Within its functioning pathways it interacts importantly with the COP9 signalosome and SCF (Skp Cullin F-box containing complex) pathway indicating its role in protein turnover and degradation. Proteins such as CUL4 and RBX1 often function in connection with DDA1 emphasizing their cooperative role in these pathways. DDA1 ensures that the cells efficiently manage their protein content affecting various cellular processes.

DDA1 presents relevance to cancer and neurodegenerative diseases. The deregulation of DDA1 expression or function can lead to impaired protein degradation contributing to the accumulation of damaged or misfolded proteins. In cancer such dysregulation may result in unchecked cell proliferation. The relationship of DDA1 with DDB1 and other CRL components highlights its importance in maintaining protein homeostasis which when disrupted can influence the progression of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 17452440, PubMed : 28302793, PubMed : 28437394, PubMed : 31686031, PubMed : 31819272). In the DCX complexes, acts as a scaffolding subunit required to stabilize the complex (PubMed : 31686031, PubMed : 31819272).
See full target information DDA1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular biology 1: PubMed36688929

2023

Biogenesis of JC polyomavirus associated extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna Morris-Love,Bethany A O'Hara,Gretchen V Gee,Aisling S Dugan,Ryan S O'Rourke,Brandon E Armstead,Benedetta Assetta,Sheila A Haley,Walter J Atwood
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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