JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB232694

Anti-DDAH2 antibody

4

(1 Review)

|

(4 Publications)

Rabbit Polyclonal DDAH2 antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Pig samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDAH2 aa 1 to C-terminus.

View Alternative Names

DDAH, G6A, NG30, DDAH2, Putative hydrolase DDAH2, DDAHII, Protein G6a, S-phase protein, DDAH-2, Inactive dimethylarginine dimethylaminohydrolase 2

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (AB232694)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (AB232694)

Paraffin-embedded human brain tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (AB232694)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (AB232694)

Paraffin-embedded human prostate tissue stained for DDAH2 using ab232694 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (AB232694)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (AB232694)

Paraffin-embedded human stomach cancer tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (AB232694)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (AB232694)

Paraffin-embedded human glioma tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (AB232694)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (AB232694)

Paraffin-embedded human colorectal cancer tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-DDAH2 antibody (AB232694)
  • WB

Supplier Data

Western blot - Anti-DDAH2 antibody (AB232694)

All lanes:

Western blot - Anti-DDAH2 antibody (ab232694) at 2 µg/mL

All lanes:

Recombinant human DDAH2 protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 29 kDa

false

Western blot - Anti-DDAH2 antibody (AB232694)
  • WB

Supplier Data

Western blot - Anti-DDAH2 antibody (AB232694)

All lanes:

Western blot - Anti-DDAH2 antibody (ab232694) at 2 µg/mL

Lane 1:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Lane 2:

Pig brain lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DDAH2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95865

style
left-column

Alternative Products

Primary Antibodies

AB184166

Anti-DDAH2 antibody [EPR15508(B)]

RabMAb|Recombinant

Western blot - Anti-DDAH2 antibody [EPR15508(B)] (AB184166)

  • 5
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15323

Anti-iNOS antibody

BOND RX™ Validated

Western blot - Anti-iNOS antibody (AB15323)

  • 4
  • 20
Primary Antibodies

AB29

Anti-PCNA antibody [PC10]

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PCNA antibody [PC10] (AB29)

  • 5
  • 72
Primary Antibodies

AB124762

Anti-p63 antibody [EPR5701]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] (AB124762)

  • 4
  • 13
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab232694 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDAH2 or Dimethylarginine Dimethylaminohydrolase 2 is an enzyme encoded by the human DDAH2 gene. With a molecular mass of approximately 30 kDa DDAH2 plays key role in the regulation of nitric oxide (NO) levels by breaking down asymmetric dimethylarginine (ADMA) an endogenous inhibitor of nitric oxide synthase (NOS). DDAH2 expression is found in a variety of tissues including the heart liver and kidney indicating its widespread functional importance in these organs.
Biological function summary

DDAH2 regulates nitric oxide production affecting processes such as vasodilation and vascular homeostasis. This enzyme also interacts in complex regulatory networks that modulate blood flow and blood pressure. By controlling ADMA concentration DDAH2 indirectly influences endothelial function and cardiovascular health. The enzyme’s role in maintaining NO levels makes it integral to processes that rely on proper endothelial signaling.

Pathways

Nitric oxide signaling and the methylarginine metabolic pathways are two significant areas involving DDAH2. In the NO signaling pathway DDAH2 impacts the synthesis of NO by modulating inhibitor levels of NOS. In the methylarginine pathway DDAH2 is closely related to the enzyme PRMT (Protein Arginine Methyltransferase) as it regulates the availability of methylarginine substrates. DDAH2’s activity harmonizes the balances within these pathways ensuring effective signaling and metabolic regulation.

Altered DDAH2 activity has connections to cardiovascular diseases and hypertension. Dysfunctional DDAH2 can lead to elevated ADMA levels which correlate with increased cardiovascular risk and impaired endothelial function. Within these diseases DDAH2’s interaction with NOS is important; improper NO production resulting from dysregulated enzyme activity affects cardiovascular health. Addressing DDAH2 malfunction holds therapeutic potential for managing related vascular disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Putative hydrolase with unknown substrate (Probable). Does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA) which acts as an inhibitor of NOS (PubMed : 21493890, PubMed : 37296100). In endothelial cells, induces expression of vascular endothelial growth factor (VEGF) via phosphorylation of the transcription factor SP1 by PKA in a process that is independent of NO and NO synthase (By similarity). Similarly, enhances pancreatic insulin secretion through SP1-mediated transcriptional up-regulation of secretagogin/SCGN, an insulin vesicle docking protein (By similarity). Upon viral infection, relocates to mitochondria where it promotes mitochondrial fission through activation of DNM1L leading to the inhibition of innate response activation mediated by MAVS (PubMed : 33850055).
See full target information DDAH2
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 84:1747-1763 PubMed38471085

2024

Whole-Genome DNA Methylation Profiling of Intrahepatic Cholangiocarcinoma Reveals Prognostic Subtypes with Distinct Biological Drivers.

Applications

Unspecified application

Species

Unspecified reactive species

Haotian Liao,Xing Chen,Haichuan Wang,Youpei Lin,Lu Chen,Kefei Yuan,Mingheng Liao,Hanyu Jiang,Jiajie Peng,Zhenru Wu,Jiwei Huang,Jiaxin Li,Yong Zeng

Nature communications 14:3392 PubMed37296100

2023

A multicentric consortium study demonstrates that dimethylarginine dimethylaminohydrolase 2 is not a dimethylarginine dimethylaminohydrolase.

Applications

Unspecified application

Species

Unspecified reactive species

Vinitha N Ragavan,Pramod C Nair,Natalia Jarzebska,Ramcharan Singh Angom,Luana Ruta,Elisa Bianconi,Silvia Grottelli,Natalia D Tararova,Daniel Ryazanskiy,Steven R Lentz,Sara Tommasi,Jens Martens-Lobenhoffer,Toshiko Suzuki-Yamamoto,Masumi Kimoto,Elena Rubets,Sarah Chau,Yingjie Chen,Xinli Hu,Nadine Bernhardt,Peter M Spieth,Norbert Weiss,Stefan R Bornstein,Debabrata Mukhopadhyay,Stefanie M Bode-Böger,Renke Maas,Ying Wang,Antonio Macchiarulo,Arduino A Mangoni,Barbara Cellini,Roman N Rodionov

Cellular and molecular neurobiology 42:2273-2288 PubMed34014421

2021

Divergent Dimethylarginine Dimethylaminohydrolase Isoenzyme Expression in the Central Nervous System.

Applications

Unspecified application

Species

Unspecified reactive species

Alena A Kozlova,Vinitha N Ragavan,Natalia Jarzebska,Iana V Lukianova,Anastasia E Bikmurzina,Elena Rubets,Toshiko Suzuki-Yamamoto,Masumi Kimoto,Arduino A Mangoni,Raul R Gainetdinov,Norbert Weiss,Michael Bauer,Alexander G Markov,Roman N Rodionov,Nadine Bernhardt

Frontiers in physiology 11:597559 PubMed33281630

2020

Upregulation of DDAH2 Limits Pulmonary Hypertension and Right Ventricular Hypertrophy During Chronic Hypoxia in Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Juliane Hannemann,Antonia Glatzel,Jonas Hillig,Julia Zummack,Udo Schumacher,Nicole Lüneburg,Lars Harbaum,Rainer Böger
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com