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AB250725

Anti-DDAH2 antibody [EPR15508(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal DDAH2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

DDAH, G6A, NG30, DDAH2, Putative hydrolase DDAH2, DDAHII, Protein G6a, S-phase protein, DDAH-2, Inactive dimethylarginine dimethylaminohydrolase 2

1 Images
Western blot - Anti-DDAH2 antibody [EPR15508(B)] - BSA and Azide free (AB250725)
  • WB

Unknown

Western blot - Anti-DDAH2 antibody [EPR15508(B)] - BSA and Azide free (AB250725)

All lanes:

Western blot - Anti-DDAH2 antibody [EPR15508(B)] (<a href='/en-us/products/primary-antibodies/ddah2-antibody-epr15508b-ab184166'>ab184166</a>) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

SW620 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 6:

Mouse brain lysate at 20 µg

Lane 7:

Rat brain lysate at 20 µg

Lane 8:

Mouse heart lysate at 20 µg

Lane 9:

Rat heart lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15508(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Primary Antibodies

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Primary Antibodies

AB180599

Anti-DDAH1 antibody [EPR13922]

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  • 4
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Primary Antibodies

AB183218

Anti-TNF alpha antibody [EPR19147]

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  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250725 is the carrier-free version of ab184166.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDAH2 or Dimethylarginine Dimethylaminohydrolase 2 is an enzyme encoded by the human DDAH2 gene. With a molecular mass of approximately 30 kDa DDAH2 plays key role in the regulation of nitric oxide (NO) levels by breaking down asymmetric dimethylarginine (ADMA) an endogenous inhibitor of nitric oxide synthase (NOS). DDAH2 expression is found in a variety of tissues including the heart liver and kidney indicating its widespread functional importance in these organs.
Biological function summary

DDAH2 regulates nitric oxide production affecting processes such as vasodilation and vascular homeostasis. This enzyme also interacts in complex regulatory networks that modulate blood flow and blood pressure. By controlling ADMA concentration DDAH2 indirectly influences endothelial function and cardiovascular health. The enzyme’s role in maintaining NO levels makes it integral to processes that rely on proper endothelial signaling.

Pathways

Nitric oxide signaling and the methylarginine metabolic pathways are two significant areas involving DDAH2. In the NO signaling pathway DDAH2 impacts the synthesis of NO by modulating inhibitor levels of NOS. In the methylarginine pathway DDAH2 is closely related to the enzyme PRMT (Protein Arginine Methyltransferase) as it regulates the availability of methylarginine substrates. DDAH2’s activity harmonizes the balances within these pathways ensuring effective signaling and metabolic regulation.

Altered DDAH2 activity has connections to cardiovascular diseases and hypertension. Dysfunctional DDAH2 can lead to elevated ADMA levels which correlate with increased cardiovascular risk and impaired endothelial function. Within these diseases DDAH2’s interaction with NOS is important; improper NO production resulting from dysregulated enzyme activity affects cardiovascular health. Addressing DDAH2 malfunction holds therapeutic potential for managing related vascular disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Putative hydrolase with unknown substrate (Probable). Does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA) which acts as an inhibitor of NOS (PubMed : 21493890, PubMed : 37296100). In endothelial cells, induces expression of vascular endothelial growth factor (VEGF) via phosphorylation of the transcription factor SP1 by PKA in a process that is independent of NO and NO synthase (By similarity). Similarly, enhances pancreatic insulin secretion through SP1-mediated transcriptional up-regulation of secretagogin/SCGN, an insulin vesicle docking protein (By similarity). Upon viral infection, relocates to mitochondria where it promotes mitochondrial fission through activation of DNM1L leading to the inhibition of innate response activation mediated by MAVS (PubMed : 33850055).
See full target information DDAH2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 12:591938 PubMed33304268

2020

1% Isoflurane and 1.2 μg/ml of Propofol: A Combination of Anesthetics That Causes the Least Damage to Hypoxic Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyue Bu,Tang Li,Di Guo,Chenyi Yang,Jinxin Wang,Xinyi Wang,Zhuo Yang,Haiyun Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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