Goat Polyclonal DDB1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human DDB1 aa 1100 to C-terminus.
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- iScience 24:1030932021Ezh2 harnesses the intranuclear actin cytoskeleton to remodel chromatin in differentiating Th cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMoran Titelbaum,Boris Brant,Daniel Baumel,Alina Burstein-Willensky,Shira Perez,Yiftah Barsheshet,Orly AvniPubMed 34622148
- Metabolism: clinical and experimental 107:1542222020DDB1 E3 ligase controls dietary fructose-induced ChREBPα stabilization and liver steatosis via CRY1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Tong et. al.PubMed 32246987
- International journal of biological sciences 16:1059-10702020Small molecule NSC1892 targets the CUL4A/4B-DDB1 interactions and causes impairment of CRL4 E3 ligases to inhibit colorectal cancer cell growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunmei Yang et. al.PubMed 32140073
- PLoS genetics 15:e10076852019Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaitao Sun et. al.PubMed 30779731
- Nature communications 9:10402018TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A.
- The Journal of cell biology 199:235-492012PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1.
- Skeletal muscle 1:152011Deregulation of the ubiquitin-proteasome system is the predominant molecular pathology in OPMD animal models and patients.
- Cancer research 69:2630-72009DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress.
- Molecular and cellular biology 28:7402-132008Cellular concentrations of DDB2 regulate dynamic binding of DDB1 at UV-induced DNA damage.
- Journal of cell science 120:2706-162007Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DDB1 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16531
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4-6 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Mouse | Dilution info 0.01-0.03 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (PubMed:14739464, PubMed:15448697, PubMed:16260596, PubMed:16407242, PubMed:16407252, PubMed:16482215, PubMed:16940174, PubMed:17079684). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (PubMed:15448697, PubMed:16260596, PubMed:16407242, PubMed:16940174). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (PubMed:15448697, PubMed:16260596, PubMed:16407242, PubMed:16940174). Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14739464, PubMed:16407252, PubMed:16482215, PubMed:17079684, PubMed:18332868, PubMed:18381890, PubMed:19966799, PubMed:22118460, PubMed:25043012, PubMed:25108355, PubMed:28886238). The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1 (PubMed:14739464, PubMed:16407252, PubMed:16482215, PubMed:17079684, PubMed:18332868, PubMed:18381890, PubMed:19966799, PubMed:22118460, PubMed:25043012, PubMed:25108355). DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage (PubMed:16473935, PubMed:16678110, PubMed:17041588, PubMed:18593899). The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair (PubMed:16473935, PubMed:16678110, PubMed:17041588, PubMed:18593899). DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER (PubMed:15882621). DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication (PubMed:17041588). DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR) (PubMed:12732143). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207). DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver (By similarity). By acting on TET dioxygenses, essential for oocyte maintenance at the primordial follicle stage, hence essential for female fertility (By similarity). Maternal factor required for proper zygotic genome activation and genome reprogramming (By similarity).
Alternative names
XAP1, DDB1, DNA damage-binding protein 1, DDB p127 subunit, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-damaged DNA-binding factor, UV-damaged DNA-binding protein 1, XPE-binding factor, Xeroderma pigmentosum group E-complementing protein, DDBa, XAP-1, UV-DDB 1, XPE-BF, XPCe
Recommended products
Goat Polyclonal DDB1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human DDB1 aa 1100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DDB1 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16531
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE).
DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DDB1 also known as Damage-Specific DNA Binding Protein 1 and DDB00A acts mechanically by recognizing and binding to DNA damage sites particularly UV-induced lesions. It functions as part of the UV-damaged DNA-binding protein complex. DDB1 has a molecular weight of approximately 127 kDa. It expresses in various tissues prominently in cells that are actively cycling. The protein often cooperates with other proteins to promote DNA repair and maintenance of genomic stability.
- Biological function summary
In terms of cellular processes beyond DDB1's immediate interactions it plays a critical role in DNA repair mechanisms and the cell cycle. DDB1 is part of the larger CUL4-DDB1 ubiquitin ligase complex which targets specific proteins for ubiquitination and subsequent degradation therefore facilitating DNA repair and regulating cell cycle progression. This ability to target damaged proteins or signaling errors for removal helps maintain cellular health and preserve genetic information integrity.
- Pathways
DDB1 is pivotal in the nucleotide excision repair pathway and the DNA damage response pathway. It interacts dynamically with proteins such as XPC (xeroderma pigmentosum group C) to initiate repair mechanisms ensuring the proper removal of damaged DNA sections. Moreover DDB1's role in ubiquitination connects it to pathways regulating protein turnover and cellular homeostasis interacting with the ubiquitin-proteasome system which is important for controlling protein degradation.
- Associated diseases and disorders
DDB1's malfunction or misregulation associates with conditions like xeroderma pigmentosum due to its role in DNA damage repair. It is also linked to certain cancer types where DNA repair deficiencies contribute to uncontrolled cell proliferation. Through these diseases DDB1 often interacts with proteins such as BRWD3 which may modulate DDB1's activity or stability influencing the disease outcomes by affecting the DNA repair capacity or recognizing specific proteins for degradation.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab9194)
Left panel: Immunohistochemical analysis of paraffin-embedded human cortex tissue labelling DDB1 with ab9194 at 4μg/ml. Heat mediated antigen retrieval with citrate buffer pH 6, HRP-staining.
Right panel: Immunohistochemical analysis of paraffin-embedded human cortex tissue with no primary antibody.
Western blot - Anti-DDB1 antibody (ab9194)
Primary incubation for 1 hour. Detected by chemiluminescence. RIPA buffer used.
All lanes: Western blot - Anti-DDB1 antibody (ab9194) at 0.01 µg/mL
Lane 1: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 35 µg
Lane 2: NSO (Murine myeloma cells) whole cell lysate at 35 µg
Predicted band size: 127 kDa
Observed band size: 140 kDa, 150 kDa
Western blot - Anti-DDB1 antibody (ab9194)
Primary incubation for 1 hour. Detected by chemiluminescence. RIPA buffer used.
All lanes: Western blot - Anti-DDB1 antibody (ab9194) at 1 µg/mL
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 35 µg
Lane 2: Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg
Predicted band size: 127 kDa
Observed band size: 140 kDa
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