Rabbit Polyclonal DDB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDB1 aa 700 to C-terminus.
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Publications
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- Experimental biology and medicine (Maywood, N.J.) 248:217-2312023Weighted gene co-expression network analysis reveals the hub genes associated with pulmonary hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengyan Wang,Dejun Sun,Chuanchuan Liu,Yong Guo,Jie Ma,Ri-Li Ge,Sen CuiPubMed 36740764
- Life (Basel, Switzerland) 12:2022Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuya Kusama,Rulan Bai,Yuta Matsuno,Atsushi Ideta,Toshihiro Sakurai,Kentaro Nagaoka,Masatoshi Hori,Kazuhiko ImakawaPubMed 35207596
- Journal of virology 96:e01360212021Proteomic Analysis of Nuclear Hepatitis B Virus Relaxed Circular DNA-Associated Proteins Identifies UV-Damaged DNA Binding Protein as a Host Factor Involved in Covalently Closed Circular DNA Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander L Marchetti,Hu Zhang,Elena S Kim,Xiaoyang Yu,Sunbok Jang,Mu Wang,Haitao GuoPubMed 34705558
- Scientific reports 10:40122020Genome-wide screening reveals a role for subcellular localization of CRBN in the anti-myeloma activity of pomalidomide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShumpei Tateno et. al.PubMed 32132601
- Nature communications 9:2592018The protective role of DOT1L in UV-induced melanomagenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Zhu et. al.PubMed 29343685
- Nucleic acids research 40:11404-152012Phosphorylation of PNKP by ATM prevents its proteasomal degradation and enhances resistance to oxidative stress.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DDB1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16531
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/10000 | Notes - |
Species Rat | Dilution info 1/500 - 1/10000 | Notes - |
Species Human | Dilution info 1/500 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (PubMed:14739464, PubMed:15448697, PubMed:16260596, PubMed:16407242, PubMed:16407252, PubMed:16482215, PubMed:16940174, PubMed:17079684). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (PubMed:15448697, PubMed:16260596, PubMed:16407242, PubMed:16940174). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (PubMed:15448697, PubMed:16260596, PubMed:16407242, PubMed:16940174). Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14739464, PubMed:16407252, PubMed:16482215, PubMed:17079684, PubMed:18332868, PubMed:18381890, PubMed:19966799, PubMed:22118460, PubMed:25043012, PubMed:25108355, PubMed:28886238). The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1 (PubMed:14739464, PubMed:16407252, PubMed:16482215, PubMed:17079684, PubMed:18332868, PubMed:18381890, PubMed:19966799, PubMed:22118460, PubMed:25043012, PubMed:25108355). DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage (PubMed:16473935, PubMed:16678110, PubMed:17041588, PubMed:18593899). The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair (PubMed:16473935, PubMed:16678110, PubMed:17041588, PubMed:18593899). DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER (PubMed:15882621). DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication (PubMed:17041588). DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR) (PubMed:12732143). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207). DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver (By similarity). By acting on TET dioxygenses, essential for oocyte maintenance at the primordial follicle stage, hence essential for female fertility (By similarity). Maternal factor required for proper zygotic genome activation and genome reprogramming (By similarity).
Alternative names
XAP1, DDB1, DNA damage-binding protein 1, DDB p127 subunit, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-damaged DNA-binding factor, UV-damaged DNA-binding protein 1, XPE-binding factor, Xeroderma pigmentosum group E-complementing protein, DDBa, XAP-1, UV-DDB 1, XPE-BF, XPCe
Recommended products
Rabbit Polyclonal DDB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDB1 aa 700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DDB1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16531
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DDB1 also known as Damage-Specific DNA Binding Protein 1 and DDB00A acts mechanically by recognizing and binding to DNA damage sites particularly UV-induced lesions. It functions as part of the UV-damaged DNA-binding protein complex. DDB1 has a molecular weight of approximately 127 kDa. It expresses in various tissues prominently in cells that are actively cycling. The protein often cooperates with other proteins to promote DNA repair and maintenance of genomic stability.
- Biological function summary
In terms of cellular processes beyond DDB1's immediate interactions it plays a critical role in DNA repair mechanisms and the cell cycle. DDB1 is part of the larger CUL4-DDB1 ubiquitin ligase complex which targets specific proteins for ubiquitination and subsequent degradation therefore facilitating DNA repair and regulating cell cycle progression. This ability to target damaged proteins or signaling errors for removal helps maintain cellular health and preserve genetic information integrity.
- Pathways
DDB1 is pivotal in the nucleotide excision repair pathway and the DNA damage response pathway. It interacts dynamically with proteins such as XPC (xeroderma pigmentosum group C) to initiate repair mechanisms ensuring the proper removal of damaged DNA sections. Moreover DDB1's role in ubiquitination connects it to pathways regulating protein turnover and cellular homeostasis interacting with the ubiquitin-proteasome system which is important for controlling protein degradation.
- Associated diseases and disorders
DDB1's malfunction or misregulation associates with conditions like xeroderma pigmentosum due to its role in DNA damage repair. It is also linked to certain cancer types where DNA repair deficiencies contribute to uncontrolled cell proliferation. Through these diseases DDB1 often interacts with proteins such as BRWD3 which may modulate DDB1's activity or stability influencing the disease outcomes by affecting the DNA repair capacity or recognizing specific proteins for degradation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)
Immunohistochemical analysis of paraffin-embedded Human Cal27 Xenograft , using ab97522 at 1/500 dilution.
Western blot - Anti-DDB1 antibody (ab97522)
5% SDS-PAGE
All lanes: Western blot - Anti-DDB1 antibody (ab97522) at 1/1000 dilution
Lane 1: PC-12 whole cell extracts at 30 µg
Lane 2: Rat2 whole cell extract at 30 µg
Predicted band size: 127 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody (ab97522)
ab97522 at 1/200 dilution staining DDB1 in Paraformaldehyde fixed HeLa cells. Lower image is merged with DNA probe.
Western blot - Anti-DDB1 antibody (ab97522)
5% SDS PAGE
All lanes: Western blot - Anti-DDB1 antibody (ab97522) at 1/5000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 127 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)
Paraffin embedded mouse prostate tissue stained for DDB1 using ab97522 at 1/500 dilutions in immunohistochemical analysis.
Western blot - Anti-DDB1 antibody (ab97522)
7.5% SDS-PAGE.
All lanes: Western blot - Anti-DDB1 antibody (ab97522) at 1/1000 dilution
All lanes: NIH/3T3 whole cell lysate at 30 µg
Predicted band size: 127 kDa
Western blot - Anti-DDB1 antibody (ab97522)
5% SDS-PAGE
All lanes: Western blot - Anti-DDB1 antibody (ab97522) at 1/1000 dilution
Lane 1: HEK-293T whole cell extract at 30 µg
Lane 2: A431 whole cell extract at 30 µg
Lane 3: HeLa whole cell extract at 30 µg
Lane 4: HepG2 whole cell extract at 30 µg
Predicted band size: 127 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)
Paraffin embedded rat fore brain tissue stained for DDB1 using ab97522 at 1/500 dilutions in immunohistochemical analysis.
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