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AB97522

Anti-DDB1 antibody

4

(4 Reviews)

|

(6 Publications)

Rabbit Polyclonal DDB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDB1 aa 700 to C-terminus.

View Alternative Names

XAP1, DDB1, DNA damage-binding protein 1, DDB p127 subunit, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-damaged DNA-binding factor, UV-damaged DNA-binding protein 1, XPE-binding factor, Xeroderma pigmentosum group E-complementing protein, DDBa, XAP-1, UV-DDB 1, XPE-BF, XPCe

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (AB97522)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (AB97522)

Immunohistochemical analysis of paraffin-embedded Human Cal27 Xenograft , using ab97522 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody (AB97522)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody (AB97522)

ab97522 at 1/200 dilution staining DDB1 in Paraformaldehyde fixed HeLa cells. Lower image is merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (AB97522)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (AB97522)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for DDB1 protein at nucleus with ab97522 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (AB97522)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (AB97522)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for DDB1 protein at nucleus with ab97522 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (AB97522)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (AB97522)

Paraffin embedded mouse prostate tissue stained for DDB1 using ab97522 at 1/500 dilutions in immunohistochemical analysis.

Western blot - Anti-DDB1 antibody (AB97522)
  • WB

Supplier Data

Western blot - Anti-DDB1 antibody (AB97522)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-DDB1 antibody (ab97522) at 1/10000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 127 kDa

false

Western blot - Anti-DDB1 antibody (AB97522)
  • WB

Supplier Data

Western blot - Anti-DDB1 antibody (AB97522)

5% SDS-PAGE

All lanes:

Western blot - Anti-DDB1 antibody (ab97522) at 1/1000 dilution

Lane 1:

HEK-293T whole cell extract at 30 µg

Lane 2:

A431 whole cell extract at 30 µg

Lane 3:

HeLa whole cell extract at 30 µg

Lane 4:

HepG2 whole cell extract at 30 µg

Predicted band size: 127 kDa

false

Western blot - Anti-DDB1 antibody (AB97522)
  • WB

Supplier Data

Western blot - Anti-DDB1 antibody (AB97522)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-DDB1 antibody (ab97522) at 1/1000 dilution

All lanes:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 127 kDa

false

Western blot - Anti-DDB1 antibody (AB97522)
  • WB

Supplier Data

Western blot - Anti-DDB1 antibody (AB97522)

5% SDS-PAGE

All lanes:

Western blot - Anti-DDB1 antibody (ab97522) at 1/1000 dilution

Lane 1:

PC-12 whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extract at 30 µg

Predicted band size: 127 kDa

false

Western blot - Anti-DDB1 antibody (AB97522)
  • WB

Supplier Data

Western blot - Anti-DDB1 antibody (AB97522)

5% SDS PAGE

All lanes:

Western blot - Anti-DDB1 antibody (ab97522) at 1/5000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 127 kDa

false

Western blot - Anti-DDB1 antibody (AB97522)
  • WB

Unknown

Western blot - Anti-DDB1 antibody (AB97522)

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-DDB1 antibody (ab97522) at 1/1000 dilution

All lanes:

NIH/3T3 whole cell lysate at 30 µg

Predicted band size: 127 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DDB1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16531

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody [EPR6089] - BSA and Azide free (AB239941)

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDB1 also known as Damage-Specific DNA Binding Protein 1 and DDB00A acts mechanically by recognizing and binding to DNA damage sites particularly UV-induced lesions. It functions as part of the UV-damaged DNA-binding protein complex. DDB1 has a molecular weight of approximately 127 kDa. It expresses in various tissues prominently in cells that are actively cycling. The protein often cooperates with other proteins to promote DNA repair and maintenance of genomic stability.
Biological function summary

In terms of cellular processes beyond DDB1's immediate interactions it plays a critical role in DNA repair mechanisms and the cell cycle. DDB1 is part of the larger CUL4-DDB1 ubiquitin ligase complex which targets specific proteins for ubiquitination and subsequent degradation therefore facilitating DNA repair and regulating cell cycle progression. This ability to target damaged proteins or signaling errors for removal helps maintain cellular health and preserve genetic information integrity.

Pathways

DDB1 is pivotal in the nucleotide excision repair pathway and the DNA damage response pathway. It interacts dynamically with proteins such as XPC (xeroderma pigmentosum group C) to initiate repair mechanisms ensuring the proper removal of damaged DNA sections. Moreover DDB1's role in ubiquitination connects it to pathways regulating protein turnover and cellular homeostasis interacting with the ubiquitin-proteasome system which is important for controlling protein degradation.

DDB1's malfunction or misregulation associates with conditions like xeroderma pigmentosum due to its role in DNA damage repair. It is also linked to certain cancer types where DNA repair deficiencies contribute to uncontrolled cell proliferation. Through these diseases DDB1 often interacts with proteins such as BRWD3 which may modulate DDB1's activity or stability influencing the disease outcomes by affecting the DNA repair capacity or recognizing specific proteins for degradation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (PubMed : 14739464, PubMed : 15448697, PubMed : 16260596, PubMed : 16407242, PubMed : 16407252, PubMed : 16482215, PubMed : 16940174, PubMed : 17079684). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (PubMed : 15448697, PubMed : 16260596, PubMed : 16407242, PubMed : 16940174). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (PubMed : 15448697, PubMed : 16260596, PubMed : 16407242, PubMed : 16940174). Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 14739464, PubMed : 16407252, PubMed : 16482215, PubMed : 17079684, PubMed : 18332868, PubMed : 18381890, PubMed : 19966799, PubMed : 22118460, PubMed : 25043012, PubMed : 25108355, PubMed : 28886238). The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1 (PubMed : 14739464, PubMed : 16407252, PubMed : 16482215, PubMed : 17079684, PubMed : 18332868, PubMed : 18381890, PubMed : 19966799, PubMed : 22118460, PubMed : 25043012, PubMed : 25108355). DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage (PubMed : 16473935, PubMed : 16678110, PubMed : 17041588, PubMed : 18593899). The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair (PubMed : 16473935, PubMed : 16678110, PubMed : 17041588, PubMed : 18593899). DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER (PubMed : 15882621). DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication (PubMed : 17041588). DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR) (PubMed : 12732143). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed : 26431207). DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver (By similarity). By acting on TET dioxygenses, essential for oocyte maintenance at the primordial follicle stage, hence essential for female fertility (By similarity). Maternal factor required for proper zygotic genome activation and genome reprogramming (By similarity).
See full target information DDB1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Experimental biology and medicine (Maywood, N.J.) 248:217-231 PubMed36740764

2023

Weighted gene co-expression network analysis reveals the hub genes associated with pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Shengyan Wang,Dejun Sun,Chuanchuan Liu,Yong Guo,Jie Ma,Ri-Li Ge,Sen Cui

Life (Basel, Switzerland) 12: PubMed35207596

2022

Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuya Kusama,Rulan Bai,Yuta Matsuno,Atsushi Ideta,Toshihiro Sakurai,Kentaro Nagaoka,Masatoshi Hori,Kazuhiko Imakawa

Journal of virology 96:e0136021 PubMed34705558

2021

Proteomic Analysis of Nuclear Hepatitis B Virus Relaxed Circular DNA-Associated Proteins Identifies UV-Damaged DNA Binding Protein as a Host Factor Involved in Covalently Closed Circular DNA Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander L Marchetti,Hu Zhang,Elena S Kim,Xiaoyang Yu,Sunbok Jang,Mu Wang,Haitao Guo

Scientific reports 10:4012 PubMed32132601

2020

Genome-wide screening reveals a role for subcellular localization of CRBN in the anti-myeloma activity of pomalidomide.

Applications

Unspecified application

Species

Unspecified reactive species

Shumpei Tateno,Midori Iida,Satoshi Fujii,Tetsufumi Suwa,Miki Katayama,Haruka Tokuyama,Junichi Yamamoto,Takumi Ito,Satoshi Sakamoto,Hiroshi Handa,Yuki Yamaguchi

Nature communications 9:259 PubMed29343685

2018

The protective role of DOT1L in UV-induced melanomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhu,Shuyang Chen,Hongshen Wang,Chengqian Yin,Changpeng Han,Cong Peng,Zhaoqian Liu,Lixin Wan,Xiaoyang Zhang,Jie Zhang,Christine G Lian,Peilin Ma,Zhi-Xiang Xu,Sharon Prince,Tao Wang,Xiumei Gao,Yujiang Shi,Dali Liu,Min Liu,Wenyi Wei,Zhi Wei,Jingxuan Pan,Yongjun Wang,Zhenyu Xuan,Jay Hess,Nicholas K Hayward,Colin R Goding,Xiang Chen,Jun Zhou,Rutao Cui

Nucleic acids research 40:11404-15 PubMed23042680

2012

Phosphorylation of PNKP by ATM prevents its proteasomal degradation and enhances resistance to oxidative stress.

Applications

WB

Species

Human

Jason L Parsons,Svetlana V Khoronenkova,Irina I Dianova,Nicola Ternette,Benedikt M Kessler,Pran K Datta,Grigory L Dianov
View all publications
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Product promise

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