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Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody ab1162 is a rabbit polyclonal antibody that is used in DDDDK tag (Binds to FLAG® tag sequence) western blotting and immunofluorescence.

- Tried and trusted by researchers since 2002
Same trusted quality, new lower price


Images

Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB1162), expandable thumbnail
  • Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB1162), expandable thumbnail
  • Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB1162), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Polyclonal

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCELISAWB
Tag
Tested
Expected
Expected

Tested
Tested

Species

Tag

Dilution info

10 µg/mL

Notes

-

Expected
Expected

Species

Tag

Dilution info

-

Notes

As coating antibody in sELISA.

Expected
Expected

Species

Tag

Dilution info

-

Notes

Antibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope" R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope.

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Alternative names

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Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody ab1162 is a rabbit polyclonal antibody that is used in DDDDK tag (Binds to FLAG® tag sequence) western blotting and immunofluorescence.

- Tried and trusted by researchers since 2002
Same trusted quality, new lower price

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Notes

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)

    ab1162 staining DDDDK tag in hTERT-RPE1 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Saponin and blocked with 0.02% BSA for 20 minutes at 18°C. Samples were incubated with primary antibody (1/100) for 45 minutes at 18°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody.

  • Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162), expandable thumbnail

    Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)

    Immunofluorescent analysis of A293 cells labeling FLAG®-VPS35 with ab1162 at 10 μg/ml. The nuclear counter stain is DAPI (blue).

  • Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162), expandable thumbnail
    Image from Kapoor N et al., J Biol Chem. 2011 Apr 22;286(16):14073-9. Epub 2011 Jan 4. Fig 1A.; doi: 10.1074/jbc.M110.185298; April 22, 2011 The Journal of Biological Chemistry, 286, 14073-14079.

    Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)

    ab1162 staining DDDDK tag (pink) in neonatal rat cardiomyocytes transduced with Ad-TBX18/FLAG®-IRES-GFP by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and incubated with primary antibody. An Alexa Fluor®-conjugated anti-rabbit IgG was used as the secondary antibody. Nuclei were stained with DAPI (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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