Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody is a rabbit polyclonal antibody that is used in DYKDDDDK epitope tag western blot (WB), immunocytochemistry (ICC), and enzyme-linked immunosorbent assay (ELISA). Suitable for FLAG® tag samples.
- Cited in over 165 publications
- Tried and trusted by researchers since 2002
- Affinity purified
Same trusted quality, new lower price
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- Nature communications 14:71062023Petal size is controlled by the MYB73/TPL/HDA19-miR159-CKX6 module regulating cytokinin catabolism in Rosa hybrida.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeikun Jing,Feifei Gong,Guoqin Liu,Yinglong Deng,Jiaqi Liu,Wenjing Yang,Xiaoming Sun,Yonghong Li,Junping Gao,Xiaofeng Zhou,Nan MaPubMed 37925502
- Cell death & disease 14:6742023DARPins detect the formation of hetero-tetramers of p63 and p73 in epithelial tissues and in squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander Strubel,Philipp Münick,Oliver Hartmann,Apirat Chaikuad,Birgit Dreier,Jonas V Schaefer,Jakob Gebel,Christian Osterburg,Marcel Tuppi,Birgit Schäfer,Viktoria Buck,Mathias Rosenfeldt,Stefan Knapp,Andreas Plückthun,Markus E Diefenbacher,Volker DötschPubMed 37828008
- NPJ vaccines 8:1392023Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKoemchhoy Khim,Sao Puth,Kamalakannan Radhakrishnan,Tien Duc Nguyen,Youn Suhk Lee,Che-Hun Jung,Shee Eun Lee,Joon Haeng RheePubMed 37752138
- The British journal of nutrition 131:202-2132023Choline-mediated hepatic lipid homoeostasis in yellow catfish: unravelling choline's lipotropic and methyl donor functions and significance of signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Feng Song,Zhen-Yu Bai,Zhi Luo,Ling-Jiao Wang,Hua ZhengPubMed 37642130
- International journal of molecular sciences 24:2023Overexpression Extends Lifespan and Healthspan in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongxuan Li,Wei Zhang,Yunshuang Ye,Yinan Sun,Liping Yang,Guijun Chen,Kangning Chen,Sheryl Smith,Jumin ZhouPubMed 37373039
- Nature communications 14:31262023Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) family.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFanindra Kumar Deshmukh,Gili Ben-Nissan,Maya A Olshina,Maria G Füzesi-Levi,Caley Polkinghorn,Galina Arkind,Yegor Leushkin,Irit Fainer,Sarel J Fleishman,Dan Tawfik,Michal SharonPubMed 37253751
- Epigenetics & chromatin 16:182023The pattern of histone H3 epigenetic posttranslational modifications is regulated by the VRK1 chromatin kinase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva Monte-Serrano,Patricia Morejón-García,Ignacio Campillo-Marcos,Aurora Campos-Díaz,Elena Navarro-Carrasco,Pedro A LazoPubMed 37179361
- Journal of translational medicine 21:1832023APE1 promotes non-homologous end joining by initiating DNA double-strand break formation and decreasing ubiquitination of artemis following oxidative genotoxic stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Zhang,Lujie Yang,Han Gao,Xunjie Kuang,He Xiao,Chen Yang,Yi Cheng,Lei Zhang,Xin Guo,Yong Zhong,Mengxia LiPubMed 36894994
- International journal of molecular sciences 24:2023VRK1 Kinase Activity Modulating Histone H4K16 Acetylation Inhibited by SIRT2 and VRK-IN-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva Monte-Serrano,Pedro A LazoPubMed 36902348
- NPJ biofilms and microbiomes 9:102023Cell specialization in cyanobacterial biofilm development revealed by expression of a cell-surface and extracellular matrix protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlona Frenkel,Eli Zecharia,Daniel Gómez-Pérez,Eleonora Sendersky,Yevgeni Yegorov,Avi Jacob,Jennifer I C Benichou,York-Dieter Stierhof,Rami Parnasa,Susan S Golden,Eric Kemen,Rakefet SchwarzPubMed 36864092
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Consider this alternative
Reactivity data
Select an application| ICC | ELISA | WB | |
|---|---|---|---|
| Tag | Tested | Expected | Expected |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 10 µg/mL | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes As coating antibody in sELISA. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes Antibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope" R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope. |
Associated Products
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Alternative names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Recommended products
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody is a rabbit polyclonal antibody that is used in DYKDDDDK epitope tag western blot (WB), immunocytochemistry (ICC), and enzyme-linked immunosorbent assay (ELISA). Suitable for FLAG® tag samples.
- Cited in over 165 publications
- Tried and trusted by researchers since 2002
- Affinity purified
Same trusted quality, new lower price
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC, WB in human, mouse, rat samples.
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has been cited over 166 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has high sensitivity and specificity.
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has 13 independent reviews from customers.
DDDDK tag (Binds to FLAG® tag sequence) antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has been validated in ELISA, ICC, WB.
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) and is sold in 100 µg selling sizes.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
This image is courtesy of an anonymous customer reviewImmunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
ab1162 staining DDDDK tag in hTERT-RPE1 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Saponin and blocked with 0.02% BSA for 20 minutes at 18°C. Samples were incubated with primary antibody (1/100) for 45 minutes at 18°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody.
Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
Immunofluorescent analysis of A293 cells labeling FLAG®-VPS35 with ab1162 at 10 μg/ml. The nuclear counter stain is DAPI (blue).
Image from Kapoor N et al., J Biol Chem. 2011 Apr 22;286(16):14073-9. Epub 2011 Jan 4. Fig 1A.; doi: 10.1074/jbc.M110.185298; April 22, 2011 The Journal of Biological Chemistry, 286, 14073-14079.Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
ab1162 staining DDDDK tag (pink) in neonatal rat cardiomyocytes transduced with Ad-TBX18/FLAG®-IRES-GFP by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and incubated with primary antibody. An Alexa Fluor®-conjugated anti-rabbit IgG was used as the secondary antibody. Nuclei were stained with DAPI (blue).
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