Rabbit Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Tag samples. Cited in 5 publications.
Images
![Immunoprecipitation - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (AB236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunoprecipitation-img142952.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (AB236777)")
![Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (AB236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunocytochemistry-immunofluorescence-img19499.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (AB236777)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (AB236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97543.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (AB236777)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (AB236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104037.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (AB236777)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (AB236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56056.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (AB236777)")
Publications
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- Virus evolution 8:veab1062022High recombination rate of hepatitis C virus revealed by a green fluorescent protein reconstitution cell system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Galli,Ulrik Fahnøe,Jens BukhPubMed 35223082
- Cell death & disease 12:7082021MiR-195 inhibits the ubiquitination and degradation of YY1 by Smurf2, and induces EMT and cell permeability of retinal pigment epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu-Hua Fu,Mei-Chen Lai,Yun-Yao Zheng,Ya-Wen Sun,Jing-Jing Qiu,Fu Gui,Qian Zhang,Fei LiuPubMed 34267179
- International journal of molecular medicine 47:2021circNRIP1 facilitates keloid progression via FXR1‑mediated upregulation of miR‑503‑3p and miR‑503‑5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaolin Wang,Hang Yin,Hongmei Zhang,Tiantian WangPubMed 33649815
- Molecular microbiology 116:154-1672021Characterization of DtrJ as an IncC plasmid conjugative DNA transfer component.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSteven J Hancock,Minh-Duy Phan,Leah W Roberts,Thu Ngoc Minh Vu,Patrick N A Harris,Scott A Beatson,Mark A SchembriPubMed 33567150
- Redox biology 38:1018012020The mA reader YTHDC2 inhibits lung adenocarcinoma tumorigenesis by suppressing SLC7A11-dependent antioxidant function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLifang Ma et. al.PubMed 33232910
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Tag | Tested | Not recommended | Expected | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
1 product for Alternative Version
Alternative names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Recommended products
Rabbit Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Tag samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20018-251
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab236777 is the carrier-free version of Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunoprecipitation-img142952.jpg "Immunoprecipitation - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)")
Immunoprecipitation - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)
DDDDK tag was immunoprecipitated from 0.35 mg HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DDDDK-tagged human PFKFB3 expression vector whole cell lysate with Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T transfected with DDDDK-tagged human PFKFB3 expression vector whole cell lysate (input).
Lane 2: Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 IP in HEK-293T transfected with DDDDK-tagged human PFKFB3 expression vector whole cell lysate.Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 in HEK-293T transfected with DDDDK-tagged human PFKFB3 expression vector whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606).
All lanes: Immunoprecipitation - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606)
![Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunocytochemistry-immunofluorescence-img19499.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)")
Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling DDDDK tag with Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 dilution (green).
Confocal image showing positive staining for FLAG® on HEK-293T cells transfected with DDDDK-tagged PFKFB3 expression vector. Mouse monoclonal anti-FLAG® M2 antibody was used as a counterstain at 1/500 dilution, and Alexa Fluor® 647 Goat anti-mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) ab150115) was used as the secondary antibody only control at 1/500 dilution. The nucleus is counterstained with DAPI.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97543.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)
Immunhistochemical analysis of agarose-embedded HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DDDDK-tagged human PD-L1 expression vector labeling DDDDK tag with Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 at 1/750 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin.
Positive staining on HEK-293T cells transfected with DDDDK-tagged human PD-L1 expression vector (Panel A) is observed. No signal was detected on HEK-293T transfected with an empty vector (vector control), containing a C-terminal DDDDK tag (Panel B).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606).
Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104037.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)
Negative control: No staining on rat spleen.Immunohistochemical analysis of paraffin-embedded rat spleen tissue stained for DDDDK tag using Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 at 1/750 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606).
Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56056.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)
Negative control: No staining on mouse spleen.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue stained for DDDDK tag using Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 at 1/750 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606).
Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83235.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)
Negative control: No staining on human hepatocellular cancer.Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer tissue stained for DDDDK tag using Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 at 1/750 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606).
Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-bsa-and-azide-free-ab236777--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83235.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free (ab236777)")
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