Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody. Suitable for ICC, ICC/IF and reacts with Tag samples. Cited in 40 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-f-tag-01-ab18230--immunocytochemistry-immunofluorescence-img92735.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230)")
![Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-f-tag-01-ab18230--immunocytochemistry-img64432.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230)")
![Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-f-tag-01-ab18230--immunocytochemistry-immunofluorescence-img132696.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230)")
Publications
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- PLoS pathogens 19:e10110702023Nuclear accumulation of host transcripts during Zika Virus Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristoffer E Leon,Mir M Khalid,Ryan A Flynn,Krystal A Fontaine,Thong T Nguyen,G Renuka Kumar,Camille R Simoneau,Sakshi Tomar,David Jimenez-Morales,Mariah Dunlap,Julia Kaye,Priya S Shah,Steven Finkbeiner,Nevan J Krogan,Carolyn Bertozzi,Jan E Carette,Melanie OttPubMed 36603024
- Cell death & disease 13:7902022Phosphorylation of PFKFB4 by PIM2 promotes anaerobic glycolysis and cell proliferation in endometriosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Lu,Pengyun Qiao,Ruihai Fu,Yadi Wang,Jiayi Lu,Xi Ling,Lu Liu,Yujun Sun,Chune Ren,Zhenhai YuPubMed 36109523
- Cell death & disease 13:2172022Tumor-derived extracellular vesicles shuttle c-Myc to promote gastric cancer growth and metastasis via the KCNQ1OT1/miR-556-3p/CLIC1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBopei Li,Yeyang Chen,Liang Liang,Ye Wang,Weijia Huang,Kun Zhao,Siyu Liu,Guofei Deng,Junqiang ChenPubMed 35260554
- Molecular therapy. Nucleic acids 27:547-5612022METTL14-mediated Lnc-LSG1 m6A modification inhibits clear cell renal cell carcinoma metastasis via regulating ESRP2 ubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanyang Shen,Lifeng Ding,Zeyi Lu,Ruyue Wang,Chenhao Yu,Huan Wang,Qiming Zheng,Xuliang Wang,Wanjiang Xu,Haifeng Yu,Liwei Xu,Mingchao Wang,Shicheng Yu,Shibin Zhu,Jun Qian,Liqun Xia,Gonghui LiPubMed 35036065
- BMC biology 19:2362021Zinc antagonizes iron-regulation of tyrosine hydroxylase activity and dopamine production in Drosophila melanogaster.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuiran Xiao,Mengran Zhao,Zhihua Liu,Fan Du,Bing ZhouPubMed 34732185
- Emerging microbes & infections 10:1975-19872021Viromes and surveys of RNA viruses in camel-derived ticks revealing transmission patterns of novel tick-borne viral pathogens in Kenya.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou Zhang,Ben Hu,Bernard Agwanda,Yaohui Fang,Jun Wang,Stephen Kuria,Juan Yang,Moses Masika,Shuang Tang,Jacqueline Lichoti,Zhaojun Fan,Zhengli Shi,Sheila Ommeh,Hualin Wang,Fei Deng,Shu ShenPubMed 34570681
- ACS pharmacology & translational science 4:1628-16382021Development of Murine Leukemia Virus Integrase-Derived Peptides That Bind Brd4 Extra-Terminal Domain as Candidates for Suppression of Acute Myeloid Leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEnming Xing,Nandini Surendranathan,Xiaotian Kong,Natalie Cyberski,Jessica D Garcia,Xiaolin Cheng,Amit Sharma,Pui-Kai Li,Ross C LaruePubMed 34661079
- Cellular and molecular gastroenterology and hepatology 12:465-4872021Paraspeckle Promotes Hepatocellular Carcinoma Immune Escape by Sequestering IFNGR1 mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Zan,Xuya Zhao,Xiya Deng,Hongda Ding,Bi Wang,Minyi Lu,Zijing Wei,Zhi Huang,Shuai WangPubMed 33667716
- Journal of cellular and molecular medicine 25:2885-28992021Paeonol attenuates inflammation by confining HMGB1 to the nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJifei Miao,Jun Zhong,Jiao Lan,Sen Ye,Peng Ye,Siyan Li,Aijia You,Xianjie Chen,Xiaoyi Liu,Hui LiPubMed 33534963
- Glycobiology :2020O-GlcNAcylation of MEK2 promotes the proliferation and migration of breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaoyao Xu et. al.PubMed 33226073
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| ICC | ICC/IF | IP | |
|---|---|---|---|
| Tag | Tested | Tested | Not recommended |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 2.00000-10.00000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Alternative names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Recommended products
Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody. Suitable for ICC, ICC/IF and reacts with Tag samples. Cited in 40 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- F-tag-01
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-f-tag-01-ab18230--immunocytochemistry-immunofluorescence-img92735.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)")
Image courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
ab18230 staining DDDDK tag in Human HeLa, cervical carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% tween20 and blocked with 0.5% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 24 hours at 4°C. Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) ab8517 (1/200) was used as the secondary antibody
![Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-f-tag-01-ab18230--immunocytochemistry-img64432.jpg "Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)")
Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
(a) Fusion of nuclear protein stained with ab18230 (red)
(b) Cell nuclei stained with DAPI (blue)
(c) Merge of (a) and (b) confirming nuclear localisation of the fusion protein.![Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-f-tag-01-ab18230--immunocytochemistry-immunofluorescence-img132696.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)")
Image from Ögmundsdóttir MH et al., PLoS One. 2012;7(5):e36616. Epub 2012 May 4. Fig 1.; doi:10.1371/journal.pone.0036616; May 4, 2012, PLoS ONE 7(5): e36616.Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
Immunofluorescence analysis of transfected HEK293 cells, staining DDDDK tag with ab18230 at 1/200 dilution.
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