AB18230

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01]

Name: Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230) | Abcam
Brand: Abcam Limited
SKU: AB18230
Rating: 4 (7 reviews)

Lab Essentials
What is this?

(7 Reviews)

(50 Publications)

Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody. Suitable for ICC, ICC/IF and reacts with Tag samples. Cited in 50 publications.

View Alternative Names

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk, flag, flag tag

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230)

ICC/IF

AbReview13821****

Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230)

ab18230 staining DDDDK tag in Human HeLa, cervical carcinoma cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% tween20 and blocked with 0.5% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 24 hours at 4°C. ab8517 (1/200) was used as the secondary antibody

Image courtesy of an anonymous AbReview

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230)

Immunofluorescence analysis of transfected HEK293 cells, staining DDDDK tag with ab18230 at 1/200 dilution.

Image from Ögmundsdóttir MH et al., PLoS One. 2012;7(5):e36616. Epub 2012 May 4. Fig 1.; doi:10.1371/journal.pone.0036616; May 4, 2012, PLoS ONE 7(5): e36616.

Unknown

Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230)

(a) Fusion of nuclear protein stained with ab18230 (red)
(b) Cell nuclei stained with DAPI (blue)
(c) Merge of (a) and (b) confirming nuclear localisation of the fusion protein.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

F-tag-01

Isotype

IgG1

Carrier free

Applications

ICC, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2-10 µg/mL", "ICC-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DDDDK tag (Binds to FLAG® tag sequence)

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Publications (50)

Recent publications for all applications. Explore the full list and refine your search

Cellular oncology (Dordrecht, Netherlands) 48:67-81 PubMed38954215

2024

Suppressing SENP1 inhibits esophageal squamous carcinoma cell growth via SIRT6 SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jianmin Gu,Shaoyuan Zhang,Dong Lin,Wenhan Wang,Jinke Cheng,Quan Zheng,Hao Wang,Lijie Tan

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309140 PubMed38639399

2024

H3K4me3-Mediated FOXJ2/SLAMF8 Axis Aggravates Thrombosis and Inflammation in β2GPI/Anti-β2GPI-Treated Monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Tan,Jiao Qiao,Shuo Yang,Hongchao Liu,Qingchen Wang,Qi Liu,Weimin Feng,Liyan Cui

International journal of molecular sciences 25: PubMed38396846

2024

The Human Mutation K237_V238del in a Putative Lipid Binding Motif within the V-ATPase a2 Isoform Suggests a Molecular Mechanism Underlying Cutis Laxa.

Applications

Unspecified application

Species

Unspecified reactive species

Anh Chu,Yeqi Yao,Miroslawa Glibowicka,Charles M Deber,Morris F Manolson

Nature communications 15:438 PubMed38200007

2024

Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Ma,Yongheng Yang,Tiejian Nie,Rong Yan,Yue Si,Jing Wei,Mengyun Li,He Liu,Wei Ye,Hui Zhang,Linfeng Cheng,Liang Zhang,Xin Lv,Limin Luo,Zhikai Xu,Xijing Zhang,Yingfeng Lei,Fanglin Zhang

Microbiology spectrum 12:e0453222 PubMed38095472

2023

Orange-spotted grouper nervous necrosis virus-encoded protein A induces interferon expression via RIG-I/MDA5-MAVS-TBK1-IRF3 signaling in fish cells.

Applications

Unspecified application

Species

Unspecified reactive species

Siyou Huang,Yi Huang,Taowen Su,Runqing Huang,Lianpan Su,Yujia Wu,Shaoping Weng,Jianguo He,Junfeng Xie

Cell death and differentiation 31:53-64 PubMed37978273

2023

TRIM3 facilitates ferroptosis in non-small cell lung cancer through promoting SLC7A11/xCT K11-linked ubiquitination and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangjie Wang,Na Shen,Ziao Wang,Lei Yu,Song Yang,Yang Wang,Yu Liu,Gaohua Han,Qi Zhang

Clinics (Sao Paulo, Brazil) 78:100303 PubMed37931529

2023

Butyrate facilitates immune clearance of colorectal cancer cells by suppressing STAT1-mediated PD-L1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Yuan Tao,Yuqing Gu,Qiujie Ma

Traffic (Copenhagen, Denmark) 24:508-521 PubMed37491993

2023

Anterograde trafficking of Toll-like receptors requires the cargo sorting adaptors TMED-2 and 7.

Applications

Unspecified application

Species

Unspecified reactive species

Julia E J Holm,Sandro G Soares,Martyn F Symmons,Afiqah Saleh Huddin,Martin C Moncrieffe,Nicholas J Gay

Journal of experimental & clinical cancer research : CR 42:38 PubMed36721234

2023

HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzhi Liu,Qishan Xu,Fan Deng,Zhuojun Zheng,Jialiang Luo,Ping Wang,Jia Zhou,Xiao Lu,Liyun Zhang,Zhengliang Chen,Qifan Zhang,Qingyun Chen,Daming Zuo

PLoS pathogens 19:e1011070 PubMed36603024

2023

Nuclear accumulation of host transcripts during Zika Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Kristoffer E Leon,Mir M Khalid,Ryan A Flynn,Krystal A Fontaine,Thong T Nguyen,G Renuka Kumar,Camille R Simoneau,Sakshi Tomar,David Jimenez-Morales,Mariah Dunlap,Julia Kaye,Priya S Shah,Steven Finkbeiner,Nevan J Krogan,Carolyn Bertozzi,Jan E Carette,Melanie Ott

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01]

Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230)

Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230)

Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (AB18230)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (50)

Product promise